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Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells
Joint Authors
Feng, Li-Xin
Liu, Qian
Wang, Hong-Shen
Deng, Yin-Hao
Yan, Ping
Duan, Xiang-Jie
Peng, Jin-Cheng
Duan, Shao-Bin
Zhao, Fei
Wu, Xi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-28
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Background.
Acute kidney injury (AKI) is one of the common complications of sepsis.
Heretofore, there is no effective treatment for septic AKI.
Recent studies have revealed that besides treating hematological malignancies, human umbilical cord blood mononuclear cells (hUCBMNCs) show good therapeutic effects on other diseases.
But whether hUCBMNCs can protect against septic AKI and its underlying mechanism are unknown.
Methods.
The rat model of lipopolysaccharide- (LPS-) induced AKI was developed, and the injection of hUCBMNCs was executed to prevent and treat AKI.
ML385, a specific nuclear factor E2-related factor 2 (Nrf2) inhibitor, was used to silence Nrf2.
The cell experiments were conducted to elaborate the protective mechanism of Nrf2 pathway.
Results.
An effective model of LPS-induced AKI was established.
Compared to the rats only with LPS injection, the levels of inflammation, reactive oxygen species (ROS), and apoptosis in renal tissues after hUCBMNC injection were markedly attenuated.
Pathological examination also indicated significant remission of renal tissue injury in the LPS+MNCs group, compared to rats in the LPS group.
Transmission electron microscopy (TEM) showed that the damage of the mitochondria in the LPS+MNCs group was lighter than that in the LPS group.
Noteworthily, the renal Nrf2/HO-1 pathway was activated and autophagy was enhanced after hUCBMNC injection.
ML385 could partly reverse the renoprotective effect of hUCBMNCs, which could demonstrate that Nrf2 participated in the protection of hUCBMNCs.
Cell experiments showed that increasing the expression level of Nrf2 could alleviate LPS-induced cell injury by increasing the autophagy level and decreasing the injury of the mitochondria in HK-2 cells.
Conclusion.
All results suggest that hUCBMNCs can protect against LPS-induced AKI via the Nrf2 pathway.
Activating Nrf2 can upregulate autophagy to protect LPS-induced cell injury.
American Psychological Association (APA)
Feng, Li-Xin& Zhao, Fei& Liu, Qian& Peng, Jin-Cheng& Duan, Xiang-Jie& Yan, Ping…[et al.]. 2020. Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1205062
Modern Language Association (MLA)
Feng, Li-Xin…[et al.]. Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1205062
American Medical Association (AMA)
Feng, Li-Xin& Zhao, Fei& Liu, Qian& Peng, Jin-Cheng& Duan, Xiang-Jie& Yan, Ping…[et al.]. Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1205062
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205062