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Presenilin 1 Regulates [Ca2+]i and MitochondriaER Interaction in Cultured Rat Hippocampal Neurons
Joint Authors
Korkotian, Eduard
Meshcheriakova, Anna
Segal, Menahem
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Mutations in the presenilin 1 (PS1) gene are a major trigger of familial Alzheimer’s disease (AD), yet the mechanisms affected by mutated PS1 causing cognitive decline are not yet elucidated.
In the present study, we compared rat hippocampal neurons in culture, transfected with PS1 or with mutant (M146V) PS1 (mPS1) plasmids in several neuronal functions.
Initially, we confirmed earlier observations that mPS1-expressing neurons are endowed with fewer mature “mushroom” spines and more filopodial immature protrusions.
The correlation between calcium changes in the cytosol, mitochondria, and endoplasmic reticulum (ER) is mitigated in the mPS1 neurons, tested by the response to an abrupt increase in ambient [Ca2+]o; cytosolic [Ca2+]i is higher in the mPS1 neurons but mitochondrial [Ca2+] is lower than in control neurons.
Strikingly, mPS1-transfected neurons express higher excitability and eventual lower survival rate when exposed to the oxidative stressor, paraquat.
These results highlight an impaired calcium regulation in mPS1 neurons, resulting in a reduced ability to handle oxidative stress, which may lead to cell death and AD.
American Psychological Association (APA)
Korkotian, Eduard& Meshcheriakova, Anna& Segal, Menahem. 2019. Presenilin 1 Regulates [Ca2+]i and MitochondriaER Interaction in Cultured Rat Hippocampal Neurons. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1205095
Modern Language Association (MLA)
Korkotian, Eduard…[et al.]. Presenilin 1 Regulates [Ca2+]i and MitochondriaER Interaction in Cultured Rat Hippocampal Neurons. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1205095
American Medical Association (AMA)
Korkotian, Eduard& Meshcheriakova, Anna& Segal, Menahem. Presenilin 1 Regulates [Ca2+]i and MitochondriaER Interaction in Cultured Rat Hippocampal Neurons. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1205095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205095