Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation
Joint Authors
Yao, Weifeng
Li, Haobo
Wu, Shan
Chen, Chaojin
Han, Xue
Guan, Yu
Guan, Jianqiang
Hei, Ziqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Neutrophil infiltration plays a critical role in the pathogenesis of acute lung injury following liver transplantation (LT).
Neutrophil elastase is released from neutrophils during pulmonary polymorphonuclear neutrophil activation and sequestration.
The aim of the study was to investigate whether the inhibition of neutrophil elastase could lead to the restoration of pulmonary function following LT.
Methods.
In in vivo experiments, lung tissue and bronchoalveolar lavage fluid (BALF) were collected at 2, 4, 8, and 24 h after rats were subjected to orthotopic autologous LT (OALT), and neutrophil infiltration was detected.
Next, neutrophil elastase inhibitors, sivelestat sodium hydrate (exogenous) and serpin family B member 1 (SERPINB1) (endogenous), were administered to rats before OALT, and neutrophil infiltration, pulmonary oxidative stress, and barrier function were measured at 8 h after OALT.
Results.
Obvious neutrophil infiltration occurred from 2 h and peaked at 8 h in the lungs of rats after they were subjected to OALT, as evidenced by an increase in naphthol-positive cells, BALF neutrophil elastase activity, and lung myeloperoxidase activity.
Treatment with neutrophil elastase inhibitors, either sivelestat sodium hydrate or SERPINB1, effectively reduced lung naphthol-positive cells and BALF inflammatory cell content, increased expression of lung HO-1 and tight junction proteins ZO-1 and occludin, and increased the activity of superoxide dismutase.
Conclusion.
Neutrophil elastase inhibitors, sivelestat sodium hydrate and SERPINB1, both reduced lung neutrophil infiltration and pulmonary oxidative stress and finally restored pulmonary barrier function.
American Psychological Association (APA)
Yao, Weifeng& Han, Xue& Guan, Yu& Guan, Jianqiang& Wu, Shan& Chen, Chaojin…[et al.]. 2019. Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1205115
Modern Language Association (MLA)
Yao, Weifeng…[et al.]. Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1205115
American Medical Association (AMA)
Yao, Weifeng& Han, Xue& Guan, Yu& Guan, Jianqiang& Wu, Shan& Chen, Chaojin…[et al.]. Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1205115
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205115