Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury
Joint Authors
Ge, Yuzheng
Jia, Ruipeng
Yifan, Zhang
Benxiang, Ning
Zheng, Xu
Luwei, Xu
Liuhua, Zhou
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
To investigate the role of inflammatory reactions and oxidative stress injury in the mechanisms of ceftriaxone calcium crystal-induced acute kidney injury (AKI) both in vivo and in vitro.
Methods.
Male Sprague Dawley rats were randomly divided into five groups of ten each according to different concentrations of ceftriaxone and calcium.
Based on the levels of serum creatinine (Scr) and blood urea nitrogen (BUN), the AKI group was chosen for the subsequent experiments.
Kidney histological examination and immunohistochemistry were performed.
The expression of NLRP3 and IL-1β protein and the concentrations of oxidative stress markers such as ROS, MDA, and H2O2 in kidney tissues were estimated.
In parallel, HK-2 human renal proximal tubule cells were exposed to ceftriaxone calcium crystals.
The mRNA expression levels of NLRP3 and IL-1β and the concentrations of oxidative stress markers were evaluated.
Finally, cell viability and rat survival were also assessed.
Results.
The results showed that significantly increased Scr and BUN levels, consistent with morphological changes and kidney stones, were found in the rats that received the highest concentration of ceftriaxone (1000 mg/kg) combined with calcium (800 mg/kg).
The activation of the NLRP3 inflammasome axis and the marked elevation of MDA, H2O2, and ROS levels were observed both in vivo and in vitro.
High expression of Nrf2, HO-1, and NQO1 was also documented.
In addition, cell apoptosis and rat mortality were promoted by ceftriaxone calcium crystals.
Conclusions.
Notably, we found that ceftriaxone-induced urolithiasis was associated with a high risk of AKI and NLRP3-mediated inflammasome and oxidative stress injury were of major importance in the pathogenesis.
American Psychological Association (APA)
Yifan, Zhang& Benxiang, Ning& Zheng, Xu& Luwei, Xu& Liuhua, Zhou& Ge, Yuzheng…[et al.]. 2020. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1205128
Modern Language Association (MLA)
Yifan, Zhang…[et al.]. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1205128
American Medical Association (AMA)
Yifan, Zhang& Benxiang, Ning& Zheng, Xu& Luwei, Xu& Liuhua, Zhou& Ge, Yuzheng…[et al.]. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1205128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205128