Aralia taibaiensis Protects against IR-Induced Brain Cell Injury through the AktSIRT1FOXO3a Pathway
Joint Authors
Duan, Jia-Lin
Guo, Chao
Weng, Yan
Xi, Miao-Miao
Cui, Jia
Zheng, Hongnan
Cao, Jinyi
Qiao, Boling
Yin, Ying
Wang, Yan-Hua
Wen, Ai-dong
Wei, Guo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-12
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Background.
Saponin from Aralia taibaiensis (sAT) showed excellent antioxidative effects in several models; however, its effects on brain cells were unknown to us.
The present study was designed to evaluate the protective effects of sAT on ischemia/reperfusion- (I/R-) induced injury and clarify its mechanisms.
Methods.
In vitro, HT22 cells were pretreated with sAT and then subjected to I/R.
Apoptosis rate, mitochondrial function, and antioxidant proteins were measured.
To clarify the mechanisms, siRNA were used.
In vivo, sAT was pretreated through intragastric administration for 7 days and the I/R model was induced.
The neurobehavioral scores, infarction volumes, and some cytokines in the brain were measured.
Protein levels were investigated by Western blotting.
Results.
The results showed that sAT treatment significantly protected cells from I/R-induced cell apoptosis and mitochondrial dysfunction.
The antioxidant protein levels were increased in a dose-dependent manner.
Further study revealed that sAT induced the deacetylation and phosphorylation of PGC-1α and FOXO3a.
sAT treatment also induced the phosphorylation levels of Akt and the expression levels of SIRT1.
Using the specific targeted siRNA transfection, the interplay relationship between Akt, SIRT1, PGC-1α, and FOXO3a was verified.
Furthermore, the same protective effects were also observed in rats subjected to I/R.
Conclusion.
sAT protected brain cells from I/R-induced mitochondrial oxidative stress and dysfunction through regulating the Akt/SIRT1/FOXO3a/PGC-1α pathway.
American Psychological Association (APA)
Duan, Jia-Lin& Cui, Jia& Zheng, Hongnan& Xi, Miao-Miao& Guo, Chao& Weng, Yan…[et al.]. 2019. Aralia taibaiensis Protects against IR-Induced Brain Cell Injury through the AktSIRT1FOXO3a Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205220
Modern Language Association (MLA)
Duan, Jia-Lin…[et al.]. Aralia taibaiensis Protects against IR-Induced Brain Cell Injury through the AktSIRT1FOXO3a Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1205220
American Medical Association (AMA)
Duan, Jia-Lin& Cui, Jia& Zheng, Hongnan& Xi, Miao-Miao& Guo, Chao& Weng, Yan…[et al.]. Aralia taibaiensis Protects against IR-Induced Brain Cell Injury through the AktSIRT1FOXO3a Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205220
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205220