Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression
Joint Authors
Meng, Guannan
Wang, Meng
Zhou, Zhen
Jiang, Hong
Wang, Zhenya
Li, Shuyan
Lai, Huanzhu
Lai, Yanqiu
Deng, Jielin
Chen, Hu
Yu, Zhiyao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
In renal ischemia/reperfusion injury (RIRI), nuclear factor κB (NF-κB) initiates the expression of multiple genes involved in inflammatory disease.
Inhibition of NF-κB-mediated inducible nitric oxide synthase (iNOS) expression can ameliorate RIRI.
Vagus nerve stimulation (VNS) protects against various organs I/R injury.
The present study was designed to elucidate the protective effect of VNS on RIRI and its influence on iNOS protein expression.
Methods.
Eighteen male Sprague-Dawley rats were randomly allocated into the sham group, the I/R group, and the VNS+I/R group, 6 rats per group.
An RIRI model was induced by a right nephrectomy and blockade of the left renal pedicle vessels for 45 min.
After 6 h of reperfusion, the blood samples and renal samples were collected.
The VNS treatment was performed throughout the I/R process in the VNS+I/R group using specific parameters (20 Hz, 0.1 ms in duration, square waves) known to produce a small but reliable bradycardia.
Blood was used for evaluation of renal function and inflammatory state.
Renal injury was evaluated via TUNEL staining.
Renal samples were harvested to evaluate renal oxidative stress, NF-κB p65 levels, and iNOS protein expression.
Results.
The VNS treatment reduces serum creatinine (Cr) and blood urea nitrogen (BUN) levels.
Simultaneously, the levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1-beta (IL-1β) were significantly increased in the I/R group, but VNS treatment markedly ameliorated this inflammatory response.
Furthermore, the VNS ameliorated oxidant stress and renal injury, indicated by a decrease in 3-nitrotyrosine (3-NT) formation and MDA and MPO levels and an increase in the SOD level compared to that in the I/R group.
Finally, the VNS also significantly decreases NF-κB p65, iNOS, and nitrite/nitrate levels compared to that in the I/R group.
Conclusion.
Our findings indicate that NF-κB activation increased iNOS expression and promoted RIRI and that VNS treatment attenuated RIRI by inhibiting iNOS expression, oxidative stress, and inflammation via NF-κB inactivation.
American Psychological Association (APA)
Wang, Meng& Deng, Jielin& Lai, Huanzhu& Lai, Yanqiu& Meng, Guannan& Wang, Zhenya…[et al.]. 2020. Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1205290
Modern Language Association (MLA)
Wang, Meng…[et al.]. Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1205290
American Medical Association (AMA)
Wang, Meng& Deng, Jielin& Lai, Huanzhu& Lai, Yanqiu& Meng, Guannan& Wang, Zhenya…[et al.]. Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1205290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205290