Peptidomics Analysis Reveals Peptide PDCryab1 Inhibits Doxorubicin-Induced Cardiotoxicity
Joint Authors
Zhang, Li
Wang, Xuejun
Feng, Mengwen
Zhang, Hao
Xu, Jia
Ding, Jingjing
Cheng, Zijie
Qian, Lingmei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-23, 23 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-14
Country of Publication
Egypt
No. of Pages
23
Main Subjects
Abstract EN
Doxorubicin (DOX) is limited due to dose-dependent cardiotoxicity.
Peptidomics is an emerging field of proteomics that has attracted much attention because it can be used to study the composition and content of endogenous peptides in various organisms.
Endogenous peptides participate in various biological processes and are important sources of candidates for drug development.
To explore peptide changes related to DOX-induced cardiotoxicity and to find peptides with cardioprotective function, we compared the expression profiles of peptides in the hearts of DOX-treated and control mice by mass spectrometry.
The results showed that 236 differential peptides were identified upon DOX treatment, of which 22 were upregulated and 214 were downregulated.
Next, we predicted that 31 peptides may have cardioprotective function by conducting bioinformatics analysis on the domains of each precursor protein, the predicted score of peptide biological activity, and the correlation of each peptide with cardiac events.
Finally, we verified that a peptide (SPFYLRPPSF) from Cryab can inhibit cardiomyocyte apoptosis, reduce the production of reactive oxygen species, improve cardiac function, and ameliorate myocardial fibrosis in vitro and vivo.
In conclusion, our results showed that the expression profiles of peptides in cardiac tissue change significantly upon DOX treatment and that these differentially expressed peptides have potential cardioprotective functions.
Our study suggests a new direction for the treatment of DOX-induced cardiotoxicity.
American Psychological Association (APA)
Zhang, Li& Wang, Xuejun& Feng, Mengwen& Zhang, Hao& Xu, Jia& Ding, Jingjing…[et al.]. 2020. Peptidomics Analysis Reveals Peptide PDCryab1 Inhibits Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-23.
https://search.emarefa.net/detail/BIM-1205313
Modern Language Association (MLA)
Zhang, Li…[et al.]. Peptidomics Analysis Reveals Peptide PDCryab1 Inhibits Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-23.
https://search.emarefa.net/detail/BIM-1205313
American Medical Association (AMA)
Zhang, Li& Wang, Xuejun& Feng, Mengwen& Zhang, Hao& Xu, Jia& Ding, Jingjing…[et al.]. Peptidomics Analysis Reveals Peptide PDCryab1 Inhibits Doxorubicin-Induced Cardiotoxicity. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-23.
https://search.emarefa.net/detail/BIM-1205313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205313