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Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
Joint Authors
Gul, Rahman
Jan, Syed Umer
Faridullah, Syed
Sherani, Samiullah
Jahan, Nusrat
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the E.
intermedia to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis.
Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay.
Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids.
High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in E.
intermedia.
The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length.
The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the E.
intermedia plant was near to the reference standard ascorbic acid.
The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan.
Results showed that average alkaloid substance in E.
intermedia was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).
American Psychological Association (APA)
Gul, Rahman& Jan, Syed Umer& Faridullah, Syed& Sherani, Samiullah& Jahan, Nusrat. 2017. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1205381
Modern Language Association (MLA)
Gul, Rahman…[et al.]. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1205381
American Medical Association (AMA)
Gul, Rahman& Jan, Syed Umer& Faridullah, Syed& Sherani, Samiullah& Jahan, Nusrat. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1205381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205381