Rat Chondrocyte Inflammation and Osteoarthritis Are Ameliorated by Madecassoside

Abstract EN

As a joint disease, osteoarthritis (OA) is caused by the breakdown of subchondral bone and cartilage damage.

Inflammatory factors, such as interleukin- (IL-) 1β, mediate the progression of OA.

Madecassoside (MA), a triterpenoid component derived from the gotu kola herb (Centella asiatica), exhibits various pharmacological effects, including antioxidative and anti-inflammatory properties.

In the present study, the protective effects and possible mechanism of MA on the treatment of OA were investigated.

MA was demonstrated to significantly suppress the IL-1β-induced overexpression of matrix metalloproteinase- (MMP-) 3, MMP-13, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) and to decrease the IL-1β-induced degradation of type II collagen and sox9.

Additionally, MA was able to reduce the IL-1β-induced phosphorylation of p65 in osteoarthritic chondrocytes.

Furthermore, in a rat OA model, MA prevented cartilage degeneration and reduced the OARSI score in the MA-treated group compared with the OA group.

The present study showed that MA suppresses the nuclear factor-κB signaling pathway, reducing IL-1β-induced chondrocyte inflammation, which indicates the therapeutic potential of MA in patients with OA.