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Astaxanthin Protects Ochratoxin A-Induced Oxidative Stress and Apoptosis in the Heart via the Nrf2 Pathway
Joint Authors
Yang, Shuhua
Long, Miao
Wang, Mingyang
Guo, Yang
Li, Peng
Cui, Gengyuan
Li, Lin
Xu, Weixiang
Jiao, Danyang
Yao, Beibei
Xu, Ketao
Chen, Yueli
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study assessed the protective mechanism of astaxanthin (ASX) against ochratoxin A- (OTA-) induced cardiac injury in mice.
Four groups of mice were established: control group (0.1 mL olive oil+0.1 mL NaHCO2), OTA group (0.1 mL OTA 5 mg/kg body weight), ASX group (0.1 mL ASX 100 mg/kg body weight), and ASX + OTA group (0.1 mL ASX 100 mg/kg body weight, 2 h later, 0.1 mL OTA 5 mg/kg body weight).
The test period lasted for 27 days (7 days of dosing, 2 days of rest).
Electrocardiogram, body weight, heart weight, tissue pathology, oxidative markers (malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH)), biochemical markers (creatine kinase (CK), creatine kinase isoenzyme (CK-MB), and lactate dehydrogenase (LDH)), electron microscopy, TUNEL, and Western blot tests were used to examine the effects of OTA on myocardial injury and ASX detoxification.
The results showed that OTA exposure significantly decreased both body weight and heart weight.
OTA induced a decrease in heart rate in mice and decreased tissue concentrations of SOD, CAT, and GSH, while increasing serum concentrations of cardiac enzymes (CK, CK-MB, and LDH) and tissue MDA.
ASX improved heart rate, cardiac enzymes, and antioxidant levels in mice.
The results of tissue pathology and TUNEL assay showed that ASX protects against OTA-induced myocardial injury.
In addition, Western blot results showed that the OTA group upregulated Keap1, Bax, Caspase3, and Caspase9, while it downregulated Nrf2, HO-1, and Bcl-2 protein expression.
ASX played a protective role by changing the expression of Keap1, Nrf2, HO-1, Bax, Bcl-2, Caspase3, and Caspase9 proteins.
These results indicate that the protective mechanism of ASX on the myocardium works through the Keap1-Nrf2 signaling pathway and mitochondria-mediated apoptosis pathway.
This study provides a molecular rationale for the mechanism underlying OTA-induced myocardial injury and the protective effect of ASX on the myocardium.
American Psychological Association (APA)
Cui, Gengyuan& Li, Lin& Xu, Weixiang& Wang, Mingyang& Jiao, Danyang& Yao, Beibei…[et al.]. 2020. Astaxanthin Protects Ochratoxin A-Induced Oxidative Stress and Apoptosis in the Heart via the Nrf2 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205412
Modern Language Association (MLA)
Cui, Gengyuan…[et al.]. Astaxanthin Protects Ochratoxin A-Induced Oxidative Stress and Apoptosis in the Heart via the Nrf2 Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1205412
American Medical Association (AMA)
Cui, Gengyuan& Li, Lin& Xu, Weixiang& Wang, Mingyang& Jiao, Danyang& Yao, Beibei…[et al.]. Astaxanthin Protects Ochratoxin A-Induced Oxidative Stress and Apoptosis in the Heart via the Nrf2 Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1205412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205412