Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia
Joint Authors
Wang, Yueming
Zhao, Nannan
Xiong, Yanlei
Zhang, Jiashen
Zhao, Dongmei
Yin, Yancun
Song, Lele
Yin, Yipeng
Wang, Jing
Luan, Xiying
Xiong, Yanlian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-07
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Red blood cells (RBCs) are susceptible to sustained free radical damage during circulation, while the changes of antioxidant capacity and regulatory mechanism of RBCs under different oxygen gradients remain unclear.
Here, we investigated the changes of oxidative damage and antioxidant capacity of RBCs in different oxygen gradients and identified the underlying mechanisms using an in vitro model of the hypoxanthine/xanthine oxidase (HX/XO) system.
In the present study, we reported that the hypoxic RBCs showed much higher oxidative stress injury and lower antioxidant capacity compared with normoxic RBCs.
In addition, we found that the disturbance of the recycling process, but not de novo synthesis of glutathione (GSH), accounted for the significantly decreased antioxidant capacity of hypoxic RBCs compared to normoxic RBCs.
We further elucidated the underlying molecular mechanism by which oxidative phosphorylation of Band 3 blocked the hexose monophosphate pathway (HMP) and decreased NADPH production aggravating the dysfunction of GSH synthesis in hypoxic RBCs under oxidative conditions.
American Psychological Association (APA)
Wang, Yueming& Zhao, Nannan& Xiong, Yanlei& Zhang, Jiashen& Zhao, Dongmei& Yin, Yancun…[et al.]. 2020. Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205437
Modern Language Association (MLA)
Wang, Yueming…[et al.]. Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205437
American Medical Association (AMA)
Wang, Yueming& Zhao, Nannan& Xiong, Yanlei& Zhang, Jiashen& Zhao, Dongmei& Yin, Yancun…[et al.]. Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205437
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205437