![](/images/graphics-bg.png)
Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma
Joint Authors
Carmona, J. U.
López, Catalina
Gutiérrez, Claudia M.
Giraldo, Carlos E.
Source
Veterinary Medicine International
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants.
The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min).
PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D.
TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma.
Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations.
PDGF-BB concentrations were not statistically different between the hemoderivatives.
Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6).
Further studies should be performed to assess the potential clinical applications of these PRPs.
American Psychological Association (APA)
Gutiérrez, Claudia M.& López, Catalina& Giraldo, Carlos E.& Carmona, J. U.. 2017. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma. Veterinary Medicine International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1205465
Modern Language Association (MLA)
Gutiérrez, Claudia M.…[et al.]. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma. Veterinary Medicine International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1205465
American Medical Association (AMA)
Gutiérrez, Claudia M.& López, Catalina& Giraldo, Carlos E.& Carmona, J. U.. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma. Veterinary Medicine International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1205465
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205465