Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide
Joint Authors
Carmona, J. U.
López, Catalina
Álvarez, María E.
Ríos, Diana L.
Pérez, Jorge E.
Source
Veterinary Medicine International
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA).
However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane.
The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS).
SMEs from six horses were cultured over 96 h.
Then, SMEs were harvested for RNA extraction and quantitative gene expression analysis by RT-qPCR for nuclear factor kappa B (NFκB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP).
The 25% and 50% Lc-PRG supernatants led to downregulation of NFκB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP in SMEs.
Lr-PRG supernatants (particularly at the 50% concentration) induced downregulation of NFκB, MMP-13, ADAMTS-4, and COL1A1 and upregulation of COL2A1 and COMP.
Lr-PRG supernatants should be used for the treatment of inflammatory arthropathies in horses because they have anti-inflammatory and anabolic effects in the synovial membrane.
American Psychological Association (APA)
Carmona, J. U.& Ríos, Diana L.& López, Catalina& Álvarez, María E.& Pérez, Jorge E.. 2017. Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide. Veterinary Medicine International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1205484
Modern Language Association (MLA)
Carmona, J. U.…[et al.]. Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide. Veterinary Medicine International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1205484
American Medical Association (AMA)
Carmona, J. U.& Ríos, Diana L.& López, Catalina& Álvarez, María E.& Pérez, Jorge E.. Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide. Veterinary Medicine International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1205484
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205484