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Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium
Joint Authors
Gallego, Carolina
Martinez, Nhora María
Patiño, María del Pilar
Esquinas, Paula
Iregui, Carlos
Romero, Stefany
Source
Veterinary Medicine International
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The role of the P.
multocida lipopolysaccharide (LPS) as a putative adhesin during the early stages of infection with this bacterium in the respiratory epithelium of rabbits was investigated.
By light microscopy and double enzyme labeling of nasal septa tissues, the amount of bacteria attached to the respiratory epithelium and the amount of LPS present in goblet cells at different experimental times were estimated.
Transmission electron microscopy (TEM) and LPS labeling with colloidal gold particles were also used to determine the exact location of LPS in the cells.
Septa that were challenged with LPS of P.
multocida and 30 minutes later with P.
multocida showed more adherent bacteria and more severe lesions than the other treatments.
Free LPS was observed in the lumen of the nasal septum, forming bilamellar structures and adhering to the cilia, microvilli, cytoplasmic membrane, and cytoplasm of epithelial ciliated and goblet cells.
The above findings suggest that P.
multocida LPS plays an important role in the process of bacterial adhesion and that it has the ability of being internalized into host cells.
American Psychological Association (APA)
Gallego, Carolina& Romero, Stefany& Esquinas, Paula& Patiño, María del Pilar& Martinez, Nhora María& Iregui, Carlos. 2017. Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium. Veterinary Medicine International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1205502
Modern Language Association (MLA)
Gallego, Carolina…[et al.]. Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium. Veterinary Medicine International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1205502
American Medical Association (AMA)
Gallego, Carolina& Romero, Stefany& Esquinas, Paula& Patiño, María del Pilar& Martinez, Nhora María& Iregui, Carlos. Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium. Veterinary Medicine International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1205502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205502