![](/images/graphics-bg.png)
Acupuncture Attenuates Inflammation in Microglia of Vascular Dementia Rats by Inhibiting miR-93-Mediated TLR4MyD88NF-κB Signaling Pathway
Joint Authors
Liu, Cun-Zhi
Yang, Jing-Wen
Wang, Xue-Rui
Fisher, Marc
Huang, Jin
Su, Xin-Tong
Wang, Lu
Cao, Yan
Lin, Li-Ting
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
It is widely accepted that inflammation may contribute to cognitive impairment in patients with vascular dementia (VD).
Our prior clinical researches have reported that acupuncture can alleviate cognitive function in VD, but the underlying mechanisms are still unclear.
The purpose of this research was to explore whether acupuncture alleviates cognitive impairment by suppressing the microRNA-93- (miR-93-) mediated Toll-like receptor (TLR) signaling pathway, which triggers inflammatory responses in the central nervous system.
Methods.
VD was established by permanent bilateral common carotid artery occlusion in male Wistar rats.
Three days after operation, the rats began daily treatment with acupuncture for two weeks.
The levels of miR-93, Toll-like receptors (TLR2 and TLR4), intracellular signaling molecules (myeloid differentiation factor 88 (MyD88) and nuclear factor-kappa B (NF-κB)), and inflammatory cytokines were subsequently detected.
TLR4 colocalized with neurons, microglia, and astrocytes in the hippocampus was evaluated.
Neuroinflammation and cognitive function were determined after intracerebroventricular injection of TLR4 antagonist TAK-242 or agonist lipopolysaccharide (LPS) with or without acupuncture.
Results.
We found that acupuncture notably repressed the expression of inflammatory cytokines in the hippocampus and plasma of VD rats.
The expression of TLR4, but not TLR2, was markedly downregulated by acupuncture, accompanied by a decrease in miR-93 and MyD88/NF-κB signaling pathway activation.
The overexpression of TLR4 in microglia, but not in astrocytes and neurons, was reversed by acupuncture.
Furthermore, intracerebroventricular injection of TAK-242 had similar effects to acupuncture on inflammation and cognitive function, while LPS injection abolished the beneficial effects of acupuncture.
Conclusions.
Taken together, these findings provide evidence that acupuncture attenuates cognitive impairment associated with inflammation through inhibition of the miR-93-mediated TLR4/MyD88/NF-κB signaling pathway in experimental VD.
Acupuncture serves as a promising alternative therapy and may be an underlying TLR4 inhibitor for the treatment of VD.
American Psychological Association (APA)
Wang, Lu& Yang, Jing-Wen& Lin, Li-Ting& Huang, Jin& Wang, Xue-Rui& Su, Xin-Tong…[et al.]. 2020. Acupuncture Attenuates Inflammation in Microglia of Vascular Dementia Rats by Inhibiting miR-93-Mediated TLR4MyD88NF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1205551
Modern Language Association (MLA)
Wang, Lu…[et al.]. Acupuncture Attenuates Inflammation in Microglia of Vascular Dementia Rats by Inhibiting miR-93-Mediated TLR4MyD88NF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1205551
American Medical Association (AMA)
Wang, Lu& Yang, Jing-Wen& Lin, Li-Ting& Huang, Jin& Wang, Xue-Rui& Su, Xin-Tong…[et al.]. Acupuncture Attenuates Inflammation in Microglia of Vascular Dementia Rats by Inhibiting miR-93-Mediated TLR4MyD88NF-κB Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1205551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205551