Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1miR-130a-3pSTAT3 Pathway
Joint Authors
Wang, Li-Ning
Jiang, Xue
Ruan, Xue-lei
Xue, Yi-xue
Yang, Shuang
Shi, Mai
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-10
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
Senescence of renal tubular epithelial cells plays an important role in diabetic nephropathy, but the mechanism is unknown.
Metformin may alleviate diabetic nephropathy by reducing this senescence.
This study is aimed at clarifying the effects and mechanism of metformin on the senescence of renal tubular epithelial cells in diabetic nephropathy.
We found that metformin reduced the expression of senescence-associated gene P21 in high-glucose-induced (30 mmol/L) renal tubular epithelial cells and decreased the β-galactosidase positive staining rate (decreased 16%, p<0.01).
Metformin was able to reduce senescence by upregulating the expression of RNA-binding protein MBNL1 and miR-130a-3p and reducing STAT3 expression.
MBNL1 prolonged the half-life of miR-130a-3p, and miR-130a-3p could negatively regulate STAT3 by binding to its mRNA 3′UTR.
In db/db diabetic mice, we found an enhanced senescence level combined with low expression of MBNL1 and miR-130a-3p and high expression of STAT3 compared with db/m control mice during nephropathy development.
Meanwhile, metformin (200 mg/kg/day) could increase the expression of MBNL1 and miR-130a-3p and decreased STAT3 expression, thus reducing this senescence in db/db mice.
Our results suggest that metformin reduces the senescence of renal tubular epithelial cells in diabetic nephropathy via the MBNL1/miR-130a-3p/STAT3 pathway, which provided new ideas for the therapy of this disease.
American Psychological Association (APA)
Jiang, Xue& Ruan, Xue-lei& Xue, Yi-xue& Yang, Shuang& Shi, Mai& Wang, Li-Ning. 2020. Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1miR-130a-3pSTAT3 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205682
Modern Language Association (MLA)
Jiang, Xue…[et al.]. Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1miR-130a-3pSTAT3 Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-22.
https://search.emarefa.net/detail/BIM-1205682
American Medical Association (AMA)
Jiang, Xue& Ruan, Xue-lei& Xue, Yi-xue& Yang, Shuang& Shi, Mai& Wang, Li-Ning. Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1miR-130a-3pSTAT3 Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-22.
https://search.emarefa.net/detail/BIM-1205682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205682