MicroRNA-31-5p Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via Inactivating Cab39AMPKα Pathway
Joint Authors
Huang, Jie
Zhou, Fang
Jiang, Wan-li
Zhao, Kao-chang
Song, Heng-ya
Liu, Gao-li
Zou, Jin-jing
Zhao, Bo
Xie, Song-ping
Yuan, Wen
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-08
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Acute lung injury (ALI) and the subsequent acute respiratory distress syndrome remain devastating diseases with high mortality rates and poor prognoses among patients in intensive care units.
The present study is aimed at investigating the role and underlying mechanisms of microRNA-31-5p (miR-31-5p) on lipopolysaccharide- (LPS-) induced ALI.
Mice were pretreated with miR-31-5p agomir, antagomir, and their negative controls at indicated doses for 3 consecutive days, and then they received a single intratracheal injection of LPS (5 mg/kg) for 12 h to induce ALI.
MH-S murine alveolar macrophage cell lines were cultured to further verify the role of miR-31-5p in vitro.
For AMP-activated protein kinase α (AMPKα) and calcium-binding protein 39 (Cab39) inhibition, compound C or lentiviral vectors were used in vivo and in vitro.
We observed an upregulation of miR-31-5p in lung tissue upon LPS injection.
miR-31-5p antagomir alleviated, while miR-31-5p agomir exacerbated LPS-induced inflammation, oxidative damage, and pulmonary dysfunction in vivo and in vitro.
Mechanistically, miR-31-5p antagomir activated AMPKα to exert the protective effects that were abrogated by AMPKα inhibition.
Further studies revealed that Cab39 was required for AMPKα activation and pulmonary protection by miR-31-5p antagomir.
We provide the evidence that endogenous miR-31-5p is a key pathogenic factor for inflammation and oxidative damage during LPS-induced ALI, which is related to Cab39-dependent inhibition of AMPKα.
American Psychological Association (APA)
Jiang, Wan-li& Zhao, Kao-chang& Yuan, Wen& Zhou, Fang& Song, Heng-ya& Liu, Gao-li…[et al.]. 2020. MicroRNA-31-5p Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via Inactivating Cab39AMPKα Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205729
Modern Language Association (MLA)
Jiang, Wan-li…[et al.]. MicroRNA-31-5p Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via Inactivating Cab39AMPKα Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1205729
American Medical Association (AMA)
Jiang, Wan-li& Zhao, Kao-chang& Yuan, Wen& Zhou, Fang& Song, Heng-ya& Liu, Gao-li…[et al.]. MicroRNA-31-5p Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via Inactivating Cab39AMPKα Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1205729
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205729