Oxidized LDL Disrupts Metabolism and Inhibits Macrophage Survival by Activating a miR-9Drp1Mitochondrial Fission Signaling Pathway
Joint Authors
Zhang, Jin
Xin, Ting
Lu, Chengzhi
Wen, Jiaxin
Yan, Shuangbin
Zhang, Jing
Li, Chao
Zhang, Feng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-03
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Mitochondrial dysfunction is associated with macrophage damage, but the role of mitochondrial fission in macrophage cholesterol metabolism is not fully understood.
In this study, we explored the influences of miR-9 and mitochondrial fission on macrophage viability and cholesterol metabolism.
Macrophages were incubated with oxidized low-density lipoprotein (ox-LDL) in vitro, after which mitochondrial fission, cell viability, and cholesterol metabolism were examined using qPCR, ELISAs, and immunofluorescence.
ox-LDL treatment significantly increased Drp1-associated mitochondrial fission.
Transfection of Drp1 siRNA significantly reduced cell death, attenuated oxidative stress, and inhibited inflammatory responses in ox-LDL-treated macrophages.
Interestingly, inhibition of Drp1-related mitochondrial fission also improved cholesterol metabolism by balancing the transcription of cholesterol influx/efflux enzymes.
We also found that miR-9 was downregulated in ox-LDL-treated macrophages, and administration of a miR-9 mimic decreased Drp1 transcription and mitochondrial fission, as well as its effects.
These results indicate that signaling via the novel miR-9/Drp1/mitochondrial fission axis is a key determinant of macrophage viability and cholesterol metabolism.
American Psychological Association (APA)
Xin, Ting& Lu, Chengzhi& Zhang, Jing& Wen, Jiaxin& Yan, Shuangbin& Li, Chao…[et al.]. 2020. Oxidized LDL Disrupts Metabolism and Inhibits Macrophage Survival by Activating a miR-9Drp1Mitochondrial Fission Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1205779
Modern Language Association (MLA)
Xin, Ting…[et al.]. Oxidized LDL Disrupts Metabolism and Inhibits Macrophage Survival by Activating a miR-9Drp1Mitochondrial Fission Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1205779
American Medical Association (AMA)
Xin, Ting& Lu, Chengzhi& Zhang, Jing& Wen, Jiaxin& Yan, Shuangbin& Li, Chao…[et al.]. Oxidized LDL Disrupts Metabolism and Inhibits Macrophage Survival by Activating a miR-9Drp1Mitochondrial Fission Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1205779
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205779