Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition
Joint Authors
Xiao, Yin
Wang, Daping
Xiong, Jianyi
Ouyang, Kan
Xu, Limei
Zhang, Peng
Xu, Xiao
Liang, Yujie
Duan, Li
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-30
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Numerous biological processes are regulated by the intercellular communications arising from extracellular vesicles (EVs) released from cells.
However, the mechanisms that regulate the quantity of EV discharged have yet to be understood.
While it is known that ATP9A, a P4-ATPase, is involved in endosomal recycling, it is not clear whether it also contributes to the release of EVs and the makeup of exosomal lipids.
This study is aimed at exploring the role of human ATP9A in the process of EV release and, further, to analyze the profiles of EV lipids regulated by ATP9A.
Our results demonstrate that ATP9A is located in both the intracellular compartments and the plasma membrane.
The percentage of ceramides and sphingosine was found to be significantly greater in the control cells than in the ATP9A overexpression and ATP9A knockout groups.
However, EV release was greater in ATP9A knockout cells, indicating that ATP9A inhibits the release of EVs.
This study revealed the effects of ATP9A on the release of EVs and the lipid composition of exosomes.
American Psychological Association (APA)
Xu, Xiao& Xu, Limei& Zhang, Peng& Ouyang, Kan& Xiao, Yin& Xiong, Jianyi…[et al.]. 2020. Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205810
Modern Language Association (MLA)
Xu, Xiao…[et al.]. Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205810
American Medical Association (AMA)
Xu, Xiao& Xu, Limei& Zhang, Peng& Ouyang, Kan& Xiao, Yin& Xiong, Jianyi…[et al.]. Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205810