Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis
Joint Authors
Mouly, Vincent
Lim, Jing Jye
Abdul Karim, Norwahidah
Wan Zurinah, Wan Ngah
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-10
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Human skeletal muscle is a vital organ involved in movement and force generation.
It suffers from deterioration in mass, strength, and regenerative capacity in sarcopenia.
Skeletal muscle satellite cells are involved in the regeneration process in response to muscle loss.
Tocotrienol, an isomer of vitamin E, was reported to have a protective effect on cellular aging.
This research is aimed at determining the modulation of tocotrienol-rich fraction (TRF) on the gene expressions of stress-induced premature senescence (SIPS) human skeletal muscle myoblasts (CHQ5B).
CHQ5B cells were divided into three groups, i.e., untreated young control, SIPS control (treated with 1 mM hydrogen peroxide), and TRF-posttreated groups (24 hours of 50 μg/mL TRF treatment after SIPS induction).
The differential gene expressions were assessed using microarray, GSEA, and KEGG pathway analysis.
Results showed that TRF treatment significantly regulated the gene expressions, i.e., p53 (RRM2B, SESN1), ErbB (EREG, SHC1, and SHC3), and FoxO (MSTN, SMAD3) signalling pathways in the SIPS myoblasts compared to the SIPS control group (p<0.05).
TRF treatment modulated the proliferation capacity of SIPS myoblasts through regulation of ErbB (upregulation of expression of EREG, SHC1, and SHC3) and FoxO (downregulation of expression of MSTN and SMAD3) and maintaining the renewal of satellite cells through p53 signalling (upregulation of RRM2B and SESN1), MRF, cell cycle, and Wnt signalling pathways.
American Psychological Association (APA)
Lim, Jing Jye& Wan Zurinah, Wan Ngah& Mouly, Vincent& Abdul Karim, Norwahidah. 2019. Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1206125
Modern Language Association (MLA)
Lim, Jing Jye…[et al.]. Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1206125
American Medical Association (AMA)
Lim, Jing Jye& Wan Zurinah, Wan Ngah& Mouly, Vincent& Abdul Karim, Norwahidah. Tocotrienol-Rich Fraction (TRF) Treatment Promotes Proliferation Capacity of Stress-Induced Premature Senescence Myoblasts and Modulates the Renewal of Satellite Cells: Microarray Analysis. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1206125
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206125