Critical Evaluation of the Interaction of Reactive Oxygen and Nitrogen Species with Blood to Inform the Clinical Translation of Nonthermal Plasma Therapy
Joint Authors
Smits, Evelien
Bogaerts, Annemie
Lin, Abraham
Biscop, Eline
Breen, Colum
Butler, Stephen J.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Non-thermal plasma (NTP), an ionized gas generated at ambient pressure and temperature, has been an emerging technology for medical applications.
Through controlled delivery of reactive oxygen and nitrogen species (ROS/RNS), NTP can elicit hormetic cellular responses, thus stimulating broad therapeutic effects.
To enable clinical translation of the promising preclinical research into NTP therapy, a deeper understanding of NTP interactions with clinical substrates is profoundly needed.
Since NTP-generated ROS/RNS will inevitably interact with blood in several clinical contexts, understanding their stability in this system is crucial.
In this study, two medically relevant NTP delivery modalities were used to assess the stability of NTP-generated ROS/RNS in three aqueous solutions with increasing organic complexities: phosphate-buffered saline (PBS), blood plasma (BP), and processed whole blood.
NTP-generated RNS collectively (NO2−, ONOO−), H2O2, and ONOO− exclusively were analyzed over time.
We demonstrated that NTP-generated RNS and H2O2 were stable in PBS but scavenged by different components of the blood.
While RNS remained stable in BP after initial scavenging effects, it was completely reduced in processed whole blood.
On the other hand, H2O2 was completely scavenged in both liquids over time.
Our previously developed luminescent probe europium(III) was used for precision measurement of ONOO− concentration.
NTP-generated ONOO− was detected in all three liquids for up to at least 30 seconds, thus highlighting its therapeutic potential.
Based on our results, we discussed the necessary considerations to choose the most optimal NTP modality for delivery of ROS/RNS to and via blood in the clinical context.
American Psychological Association (APA)
Lin, Abraham& Biscop, Eline& Breen, Colum& Butler, Stephen J.& Smits, Evelien& Bogaerts, Annemie. 2020. Critical Evaluation of the Interaction of Reactive Oxygen and Nitrogen Species with Blood to Inform the Clinical Translation of Nonthermal Plasma Therapy. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206135
Modern Language Association (MLA)
Lin, Abraham…[et al.]. Critical Evaluation of the Interaction of Reactive Oxygen and Nitrogen Species with Blood to Inform the Clinical Translation of Nonthermal Plasma Therapy. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1206135
American Medical Association (AMA)
Lin, Abraham& Biscop, Eline& Breen, Colum& Butler, Stephen J.& Smits, Evelien& Bogaerts, Annemie. Critical Evaluation of the Interaction of Reactive Oxygen and Nitrogen Species with Blood to Inform the Clinical Translation of Nonthermal Plasma Therapy. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206135
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206135