PI3KAkt and ERK12 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury
Joint Authors
Jazvinšćak Jembrek, Maja
Šimić, Goran
Zubčić, Klara
Radovanović, Vedrana
Vlainić, Josipa
Oršolić, Nada
Hof, P. R.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Copper, a transition metal with essential cellular functions, exerts neurotoxic effects when present in excess by promoting production of reactive oxygen species (ROS).
The aim of the present study was to investigate potential benefits of flavonoid quercetin against copper-induced toxicity.
Results obtained with MTT assay indicate that the effects of quercetin are determined by the severity of the toxic insult.
In moderately injured P19 neuronal cells, concomitant treatment with 150 μM quercetin improved viability by preventing ROS formation, caspase-3 activation, and chromatin condensation.
Western blot analysis revealed that quercetin reduced copper-induced increase in p53 upregulated modulator of apoptosis (PUMA) expression and promoted upregulation of nucleoside diphosphate kinase NME1.
Levels of p53 and Bax proteins were not affected by both copper and quercetin.
UO126 and wortmannin, inhibitors of ERK1/2 and PI3K/Akt signalling pathways, respectively, prevented neuroprotective effects of quercetin.
In severely injured neurons, 30 μM quercetin exerted strong prooxidative action and exacerbated cytotoxic effects of copper, whereas 150 μM quercetin failed to affect neuronal survival.
These results demonstrate the dual nature of quercetin action in copper-related neurodegeneration.
Hence, they are relevant in the context of considering quercetin as a possible therapeutic for neuroprotection and imply that detailed pharmacological and toxicological studies must be carried out for natural compounds capable of acting both as antioxidants and prooxidants.
American Psychological Association (APA)
Zubčić, Klara& Radovanović, Vedrana& Vlainić, Josipa& Hof, P. R.& Oršolić, Nada& Šimić, Goran…[et al.]. 2020. PI3KAkt and ERK12 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1206172
Modern Language Association (MLA)
Zubčić, Klara…[et al.]. PI3KAkt and ERK12 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1206172
American Medical Association (AMA)
Zubčić, Klara& Radovanović, Vedrana& Vlainić, Josipa& Hof, P. R.& Oršolić, Nada& Šimić, Goran…[et al.]. PI3KAkt and ERK12 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1206172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206172