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Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APPPS1 Transgenic Alzheimer’s Disease Mice
Joint Authors
Liu, Sulai
Yu, Nanhui
Huang, Yongpan
Jiang, Yu
Zou, Lianhong
Liu, Xiehong
Chen, Fang
Luo, Jun
Zhu, Yimin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Alzheimer’s disease (AD) is the most common cause of dementia among senior citizen.
Ganoderma lucidum triterpenoids (GLTs) have nutritional health benefits and has been shown to promote health and longevity, but a protective effect of GLTs on AD damage has not yet been reported.
The objective of this research was to elucidate the phylactic effect of GLTs on AD model mice and cells and to explore its underlying mechanisms.
Morris water maze (MWM) test was conducted to detect changes in the cognitive function of mice.
Hematoxylin-eosin (HE) staining was applied to observe pathological changes in the hippocampus.
Silver nitrate staining was applied to observe the hippocampal neuronal tangles (NFTs).
Apoptosis of the hippocampal neurons in mouse brain tissue was determined by TUNEL staining.
The expression levels of apoptosis-related protein Bcl2, Bax, and caspase 3/cleaved caspase 3; antioxidative protein Nrf2, NQO1, and HO1; and ROCK signaling pathway-associated proteins ROCK2 and ROCK1 were measured by western blot.
In vivo experiments show that 5-month-old APP/PS1 mice appeared to have impaired acquisition of spatial learning and GLTs could reduce cognitive impairment in AD mice.
Compared to normal mice, the hippocampus of APP/PS1 mouse’s brains was severely damaged, while GLTs could alleviate this symptom by inhibiting apoptosis, relieving oxidative damage, and inactivating the ROCK signaling pathway.
In in vitro cell experiments, Aβ25-35 was applied to induce hippocampal neurons into AD model cells.
GLTs promoted cell proliferation, facilitated superoxide dismutase (SOD) expression, and inhibited malondialdehyde (MDA) and lactic dehydrogenase (LDH) expression of neurons.
Our study highlights that GLTs improve cognitive impairment, alleviate neuronal damage, and inhibit apoptosis in the hippocampus tissues and cells in AD through inhibiting the ROCK signaling pathway.
American Psychological Association (APA)
Yu, Nanhui& Huang, Yongpan& Jiang, Yu& Zou, Lianhong& Liu, Xiehong& Liu, Sulai…[et al.]. 2020. Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APPPS1 Transgenic Alzheimer’s Disease Mice. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1206188
Modern Language Association (MLA)
Yu, Nanhui…[et al.]. Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APPPS1 Transgenic Alzheimer’s Disease Mice. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1206188
American Medical Association (AMA)
Yu, Nanhui& Huang, Yongpan& Jiang, Yu& Zou, Lianhong& Liu, Xiehong& Liu, Sulai…[et al.]. Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APPPS1 Transgenic Alzheimer’s Disease Mice. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1206188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206188