Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
Joint Authors
Qi, Dake
Tong, Haibin
Wu, Hong
Wang, Xinzhou
Gao, Shuibo
Dai, Liping
Gao, Haixia
Lei, Zhen
Han, Yongjun
Wang, Zhentao
Han, Lihua
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The Yiqi-Huoxue granule (YQHX) is a traditional Chinese medication widely used in the therapy of the traditional Chinese medicine diagnosis “Qi deficiency” or “blood stasis” in China.
Both these symptoms are related to inflammation, but the mechanisms of YQHX against inflammation are largely unknown.
Thus, our present study investigated the effects of YQHX on regulating inflammatory responses induced by lipopolysaccharides (LPS) in HUVECs.
Our data found that YQHX remarkably inhibits the production of prothrombotic factors, plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF), while it upregulates the protein expression of Kruppel-like factor 2 (KLF2).
The increase in PAI-1 and TF was significantly attenuated through a transgenic knockdown in KLF2 with a Lenti-shKLF2 vector.
YQHX also decreases the phosphorylation of nuclear factor-κB (NF-κB) p65 and IκB following LPS stimulation, and it effectively suppresses PAI-1 and TF via a NF-κB-dependent mechanism.
Taken together, our results suggest that YQHX provides a notable antithrombotic activity via regulating the KLF2 expression and NF-κB signaling pathway in HUVECs.
The KLF2 and NF-κB may be potential therapeutic targets for interventions of inflammation associated with atherosclerosis.
American Psychological Association (APA)
Wu, Hong& Wang, Xinzhou& Gao, Shuibo& Dai, Liping& Tong, Haibin& Gao, Haixia…[et al.]. 2019. Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1206392
Modern Language Association (MLA)
Wu, Hong…[et al.]. Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1206392
American Medical Association (AMA)
Wu, Hong& Wang, Xinzhou& Gao, Shuibo& Dai, Liping& Tong, Haibin& Gao, Haixia…[et al.]. Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1206392
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206392