Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress
Joint Authors
Chi, Xinjin
Cai, Jun
Lv, Peibiao
Chen, Tufeng
Liu, Peibin
Zheng, Lei
Tian, Jingling
Tan, Fan
Chen, Jiaxin
Deng, Yingqing
Li, Jun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Patients with orthotopic liver transplantation (OLT) frequently develop acute gut injury (AGI), and dexmedetomidine (Dex) has been reported to exert a protective effect against AGI.
We investigated whether Dex protects against AGI through antioxidative stress effects by the Nrf2/HO-1 antioxidative signaling pathway.
Rats were randomly allocated into a sham group and six orthotopic autologous liver transplantation (OALT) groups receiving different doses of Dex together with/without α2-adrenergic receptor (AR) blockers.
Intestinal tissues were collected to visualize the barrier damage and to measure the indexes of oxidative stress.
For in vitro studies, rat intestinal recess epithelial cells (IEC-6) underwent hypoxia/reoxygenation (H/R), and the protective role of Dex was evaluated after α2A-AR siRNA silencing.
OALT resulted in increased oxidative stress, significant intestinal injury, and barrier dysfunction.
Dex attenuated OALT-induced oxidative stress and intestinal injury, which was abolished by the pretreatment with the nonspecific α2A-AR siRNA blocker atipamezole and the specific α2A-AR siRNA blocker BRL-44408, but not by the specific 2B/C-AR siRNA blocker ARC239.
Silencing of α2A-AR siRNA also attenuated the protective role of Dex on alleviating oxidative stress in IEC-6 cells subjected to H/R.
Dex exerted its protective effects by activating Nrf2/HO-1 antioxidative signaling.
Collectively, Dex attenuates OALT-induced AGI via α2A-AR-dependent suppression of oxidative stress, which might be a novel potential therapeutic target for OALT-induced AGI.
American Psychological Association (APA)
Lv, Peibiao& Chen, Tufeng& Liu, Peibin& Zheng, Lei& Tian, Jingling& Tan, Fan…[et al.]. 2019. Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1206395
Modern Language Association (MLA)
Lv, Peibiao…[et al.]. Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1206395
American Medical Association (AMA)
Lv, Peibiao& Chen, Tufeng& Liu, Peibin& Zheng, Lei& Tian, Jingling& Tan, Fan…[et al.]. Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1206395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206395