Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
Joint Authors
Guissou, Innocent Pierre
Koffi, Camille
Soleti, Raffaella
Nitiema, Mathieu
Mallegol, Patricia
Belemnaba, Lazare
Ouédraogo, Noufou
Kini, Félix Bondo
Ouédraogo, Sylvin
Andriantsitohaina, Ramaroson
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension.
This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice.
Its effects on vascular function were specifically investigated.
Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl2 ex vivo on isolated aortas.
Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage.
In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day).
LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II.
Echocardiographic and kidney parameters were not affected by the different conditions.
LMAE abrogated Ang II-induced impairment of ACh-induced relaxation without affecting that of sodium nitroprusside.
LMAE also completely prevented CaCl2-induced contraction in KCl-exposed aorta ex vivo.
The extract alone did not modify superoxide (O2-) and nitric oxide (NO⋅) production in femoral arteries from control mice but significantly limited Ang II-induced O2- production.
These effects were associated with reduced expression of inducible isoform of cyclooxygenase- (COX-) 2 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase isoform NOX-2 in aortas.
Finally, phytochemical analysis showed that LMAE contains sterols, triterpenes, coumarins, and anthraquinone.
These results showed that LMAE prevents Ang II-induced hypertension and vascular dysfunction through a reduction of oxidative stress linked to COX-2 and NOX-2 pathway and inhibition of calcium entry.
This study provides pharmacological basis of the empirical use of Lannea microcarpa trunk bark extract against hypertension.
American Psychological Association (APA)
Nitiema, Mathieu& Soleti, Raffaella& Koffi, Camille& Belemnaba, Lazare& Mallegol, Patricia& Ouédraogo, Noufou…[et al.]. 2019. Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1206409
Modern Language Association (MLA)
Nitiema, Mathieu…[et al.]. Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1206409
American Medical Association (AMA)
Nitiema, Mathieu& Soleti, Raffaella& Koffi, Camille& Belemnaba, Lazare& Mallegol, Patricia& Ouédraogo, Noufou…[et al.]. Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1206409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206409