Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway
Joint Authors
Al-Khedhairy, Abdulaziz A.
Al-Massarani, Shaza M.
Siddiqui, Maqsood A.
Al-Sheddi, Ebtesam S.
Farshori, Nida N.
Al-Oqail, Mai M.
Al-Turki, Eman A.
Ahmad, Javed
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Cancer has been recognized as one of the life-threating diseases.
Breast cancer is a leading cause of mortality among women.
In spite of current developments in the therapy and diagnosis of cancer, the survival rate is still less.
Recently, plant-derived natural products gain attention as anticancer agents due to the nontoxic nature.
Therefore, the aim of present study was to investigate the anticancer capacity of corn silk extract (CSE) on human breast cancer (MCF-7) and normal human mesenchymal (hMSC-TERT4) cells.
Following 24 h treatment to corn silk extract, the cytotoxicity was assessed by MTT, NRU, and morphological assays.
The oxidative stress markers (GSH and LPO), ROS production, MMP change, and expression of apoptotic marker genes (p53, Bax, Bcl-2, caspase-3, and caspase-9) were also studied in MCF-7 cells treated at 250 to 1000 μg/ml of CSE for 24 h.
Our results showed that CSE decreased the cell viability and increased the apoptosis in a dose-dependent manner.
The level of LPO and ROS production was found significantly higher; however, GSH and MMP level was observed lower in CSE-treated MCF-7 cells.
The real-time PCR data showed a significant upregulation in p53, Bax, caspase-3, and caspase-9 and downregulation in the mRNA expression of Bcl-2 genes in MCF-7 cells exposed to CSE.
Collectively, the data from this study stated that corn silk extract induced apoptosis via the ROS-mediated mitochondrial pathway in MCF-7 cells.
American Psychological Association (APA)
Al-Oqail, Mai M.& Al-Sheddi, Ebtesam S.& Farshori, Nida N.& Al-Massarani, Shaza M.& Al-Turki, Eman A.& Ahmad, Javed…[et al.]. 2019. Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1206641
Modern Language Association (MLA)
Al-Oqail, Mai M.…[et al.]. Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1206641
American Medical Association (AMA)
Al-Oqail, Mai M.& Al-Sheddi, Ebtesam S.& Farshori, Nida N.& Al-Massarani, Shaza M.& Al-Turki, Eman A.& Ahmad, Javed…[et al.]. Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1206641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206641