Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells
Joint Authors
Huang, Xiaoyun
Zhao, Zhongyan
Xu, Zhiyu
Liu, Tao
Huang, Shixiong
Huang, Huai
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Migraine is one of the most common neurological disorders which poses significant socioeconomic burden worldwide.
Neuroinflammation and oxidative stress both play important roles in the pathogenesis of migraine.
Human urinary kallidinogenase (UK) is a tissue kallikrein derived from human urine.
Increasing evidence suggests that UK may protect against ischemic stroke, but UK’s treatment potential against migraine remains to be explored.
Immortal BV-2 murine microglial cells were treated with UK (125 nM, 250 nM, and 500 nM) and then given lipopolysaccharides (LPS, 1000 ng/mL).
Cell viability of BV-2 cells was tested by the CCK-8 assay.
Expressions of tumor necrosis factor-α (TNFα), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were examined with the ELISA method and western blot.
Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were measured to determine oxidative stress.
Our results showed that LPS administration increased the levels of proinflammatory cytokines (TNFα, PGE2, IL-6, and IL-1β) and oxidative stress (ROS and MDA) when compared with the control group and decreased significantly upon introduction with UK.
Taken together, UK treatment reduced LPS-induced neuroinflammation and oxidative stress in a dose-dependent manner, which might be a potential treatment of migraine.
American Psychological Association (APA)
Zhao, Zhongyan& Xu, Zhiyu& Liu, Tao& Huang, Shixiong& Huang, Huai& Huang, Xiaoyun. 2019. Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells. Pain Research and Management،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1207316
Modern Language Association (MLA)
Zhao, Zhongyan…[et al.]. Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells. Pain Research and Management No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1207316
American Medical Association (AMA)
Zhao, Zhongyan& Xu, Zhiyu& Liu, Tao& Huang, Shixiong& Huang, Huai& Huang, Xiaoyun. Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells. Pain Research and Management. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1207316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207316