Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences
Joint Authors
Meemongkolkiat, Thitipan
Rattanawannee, Atsalek
Chanchao, Chanpen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Knowledge of the genetic diversity of Apis spp.
is important in order to provide a better understanding of breeding strategies that relate to the conservation of wild species and colony survival of farmed species.
Here, honeybees of five Apis species were collected from 12 provinces throughout Thailand.
After DNA extraction, 28S rRNA nuclear (710 bp) and cytochrome b (cytb) mitochondrial (520 bp) gene fragments were sequenced.
Homologous sequences (nucleotide identity of over 95%) were obtained from GeneBank using the BLASTn algorithm, aligned, and analysed by maximum likelihood and Bayesian inference phylogenetics.
For 28S rRNA, a low genetic variation was detected within species (haplotype diversity ranging from 0.212 to 0.394), while 19 polymorphic sites were detected between species.
Although the relative haplotype diversity was high, a low nucleotide divergence was found (0.7%), with migratory species.
For cytb, the sequence divergence ranged from 0.24 to 3.88% within species and 7.35 to 13.07% between species.
The divergence of cytb was higher than that of 28S rRNA.
A.
cerana showed two distinct clades between Southern Thailand and the other regions.
Groups of A.
cerana (Asian cavity-nesting), A.
mellifera (European cavity-nesting), A.
dorsata (giant open-nesting), and A.
florea and A.
andreniformis (dwarf bees) were defined in the 28S rRNA and cytb tree topologies.
American Psychological Association (APA)
Meemongkolkiat, Thitipan& Rattanawannee, Atsalek& Chanchao, Chanpen. 2019. Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences. Psyche،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1207471
Modern Language Association (MLA)
Meemongkolkiat, Thitipan…[et al.]. Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences. Psyche No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1207471
American Medical Association (AMA)
Meemongkolkiat, Thitipan& Rattanawannee, Atsalek& Chanchao, Chanpen. Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences. Psyche. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1207471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207471