A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

Joint Authors

Zhao, Yajun
Xiao, E.
Lv, Wanqi
Dong, Xian
He, Linhai
Wang, Yixiang
Zhang, Yi

Source

Stem Cells International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-03-07

Country of Publication

Egypt

No. of Pages

12

Abstract EN

Mesenchymal stem cells (MSCs) possess promising potential in tissue engineering and regenerative medicine.

Previous studies demonstrated that spheroid formation of MSCs exhibited improved stemness maintenance and therapeutic potential compared with monolayer culture.

To date, various spheroid culture systems have been developed but most of them required low adhesion conditions or special equipment.

In this study, we demonstrated that inoculation of dissociated MSCs in TeSR-E8 medium could induce self-assemble spheroid formation in conventional tissue culture polystyrene dishes.

Compared with monolayer culture, adipose-derived stem cell (ADSC) spheroids enhanced the proliferation and osteogenic capability of ADSCs compared with monolayer culture.

When reseeded in normal serum-containing medium, the expression level of stemness biomarkers was even higher in spheroid-derived ADSCs than monolayer culture.

Importantly, spheroid ADSCs could effectively promote the M2 polarization of macrophages both in vitro and in vivo.

After transplantation into mouse, spheroid ADSCs improved the survival rate and significantly decreased serum levels of proinflammatory factors IL-1β and TNF-α following LPS challenge.

In summary, we developed a 3D spheroid culture system through TeSR-E8 medium without the involvement of low adhesion conditions and special equipment, which provided a practical and convenient method for spheroid formation of MSCs with great potential for stem cell clinical therapy.

American Psychological Association (APA)

Zhao, Yajun& Xiao, E.& Lv, Wanqi& Dong, Xian& He, Linhai& Wang, Yixiang…[et al.]. 2020. A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1207581

Modern Language Association (MLA)

Zhao, Yajun…[et al.]. A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1207581

American Medical Association (AMA)

Zhao, Yajun& Xiao, E.& Lv, Wanqi& Dong, Xian& He, Linhai& Wang, Yixiang…[et al.]. A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1207581

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1207581