Analysis of the CDK46 Cell Cycle Pathway in Leiomyosarcomas as a Potential Target for Inhibition by Palbociclib
Joint Authors
Möller, Peter
Böhm, Michael J.
Marienfeld, Ralf
Jäger, Daniela
Mellert, Kevin
von Witzleben, Adrian
Brüderlein, Silke
Wittau, Mathias
von Baer, Alexandra
Schultheiss, Markus
Mayer-Steinacker, Regine
Rücker, Frank G.
Bullinger, Lars
Barth, Thomas F. E.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Leiomyosarcoma (LMS) is characterized by high genomic complexity, and to date, no specific targeted therapy is available.
In a genome-wide approach, we profiled genomic aberrations in a small cohort of eight primary tumours, two relapses, and eight metastases across nine different patients.
We identified CDK4 amplification as a recurrent alteration in 5 out of 18 samples (27.8%).
It has been previously shown that the LMS cell line SK-LMS-1 has a defect in the p16 pathway and that this cell line can be inhibited by the CDK4 and CDK6 inhibitor palbociclib.
For SK-LMS-1 we confirm and for SK-UT-1 we show that both LMS cell lines express CDK4 and that, in addition, strong CDK6 expression is seen in SK-LMS-1, whereas Rb was expressed in SK-LMS-1 but not in SK-UT-1.
We confirm that inhibition of SK-LMS-1 with palbociclib led to a strong decrease in protein levels of Phospho-Rb (Ser780), a decreased cell proliferation, and G0/G1-phase arrest with decreased S/G2 fractions.
SK-UT-1 did not respond to palbociclib inhibition.
To compare these in vitro findings with patient tissue samples, a p16, CDK4, CDK6, and p-Rb immunohistochemical staining assay of a large LMS cohort (n=99 patients with 159 samples) was performed assigning a potential responder phenotype to each patient, which we identified in 29 out of 99 (29.3%) patients.
Taken together, these data show that CDK4/6 inhibitors may offer a new option for targeted therapy in a subset of LMS patients.
American Psychological Association (APA)
Böhm, Michael J.& Marienfeld, Ralf& Jäger, Daniela& Mellert, Kevin& von Witzleben, Adrian& Brüderlein, Silke…[et al.]. 2019. Analysis of the CDK46 Cell Cycle Pathway in Leiomyosarcomas as a Potential Target for Inhibition by Palbociclib. Complexity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1207638
Modern Language Association (MLA)
Böhm, Michael J.…[et al.]. Analysis of the CDK46 Cell Cycle Pathway in Leiomyosarcomas as a Potential Target for Inhibition by Palbociclib. Complexity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1207638
American Medical Association (AMA)
Böhm, Michael J.& Marienfeld, Ralf& Jäger, Daniela& Mellert, Kevin& von Witzleben, Adrian& Brüderlein, Silke…[et al.]. Analysis of the CDK46 Cell Cycle Pathway in Leiomyosarcomas as a Potential Target for Inhibition by Palbociclib. Complexity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1207638
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207638