Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin

Abstract EN

Objectives.

To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs).

Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored.

Methods.

Flow cytometry is used for cell identification.

The differentiation ability of hBM-MSCs was studied by differentiation culture.

SA-β-gal staining is used to detect cell senescence levels.

Western blot and immunofluorescence were used to determine protein expression levels.

RT-qPCR is used to detect mRNA expression levels.

Results.

Rg1 regulates the differentiation of hBM-MSCs.

Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis.

Western blot results showed that Rg1 regulated the overactivation of the β-catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3β.

GSK-3β inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3β, which in turn reduced Rg1-induced differentiation of hBM-MSCs.

Conclusion.

Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3β and regulate the mesenchymal stem cell differentiation ability.