![](/images/graphics-bg.png)
Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct
Joint Authors
Kaplan, David L.
Bender, Robert
McCarthy, Michelle
Brown, Theodore
Bukowska, Joanna
Smith, Stanley
Abbott, Rosalyn D.
Williams, Christopher
Wade, James W.
Alarcon, Andrea
Wu, Xiying
Lau, Frank
Gimble, Jeffrey M.
Frazier, Trivia
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-10
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Obesity, defined as a body mass index of 30 kg/m2 or above, has increased considerably in incidence and frequency within the United States and globally.
Associated comorbidities including cardiovascular disease, type 2 diabetes mellitus, metabolic syndrome, and nonalcoholic fatty liver disease have led to a focus on the mechanisms promoting the prevention and treatment of obesity.
Commonly utilized in vitro models employ human or mouse preadipocyte cell lines in a 2-dimensional (2D) format.
Due to the structural, biochemical, and biological limitations of these models, increased attention has been placed on “organ on a chip” technologies for a 3-dimensional (3D) culture.
Herein, we describe a method employing cryopreserved primary human stromal vascular fraction (SVF) cells and a human blood product-derived biological scaffold to create a 3D adipose depot in vitro.
The “fat-on-chip” 3D cultures have been validated relative to 2D cultures based on proliferation, flow cytometry, adipogenic differentiation, confocal microscopy/immunofluorescence, and functional assays (adipokine secretion, glucose uptake, and lipolysis).
Thus, the in vitro culture system demonstrates the critical characteristics required for a humanized 3D white adipose tissue (WAT) model.
American Psychological Association (APA)
Bender, Robert& McCarthy, Michelle& Brown, Theodore& Bukowska, Joanna& Smith, Stanley& Abbott, Rosalyn D.…[et al.]. 2020. Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct. Stem Cells International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1207723
Modern Language Association (MLA)
Bender, Robert…[et al.]. Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct. Stem Cells International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1207723
American Medical Association (AMA)
Bender, Robert& McCarthy, Michelle& Brown, Theodore& Bukowska, Joanna& Smith, Stanley& Abbott, Rosalyn D.…[et al.]. Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1207723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207723