Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation
Joint Authors
Zhang, Xiaojun
Li, Huanhuan
Jiang, Xuheng
Fang, Xuanyu
Xie, Fangke
Zhang, Ji
Huang, Xiaofei
Ge, Hongfei
Yu, Anyong
Hu, Quan
Zhong, Jun
Zhang, Chao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-16
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Although neural stem cells (NSCs) could migrate towards lesions after central nervous system (CNS) injury, the migration ability always is restricted due to the disturbed composition and density of the adhesion ligands and extracellular matrix (ECM) gradient after CNS injury.
To date, various methods have been developed to enhance NSC migration and a number of factors, which are affecting NSC migration potential, have been identified.
Here, primary NSCs were cultured and the expression of actin alpha 2 (ACTA2) in NSCs was determined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining.
Next, the role of ACTA2 in regulating NSC migration and the potential mechanism was explored.
Our results demonstrated that ACTA2 expressed in NSCs.
Meanwhile, downregulated ACTA2 using siRNA inhibited NSC migration through hindering actin filament polymerization via increasing RhoA expression and decreasing Rac1 expression.
The present study might enrich the basic knowledge of ACTA2 in NSC migration and open an avenue for enhancing NSC migration potential, subsequently providing an intervention target for functional recovery after CNS injury.
American Psychological Association (APA)
Zhang, Ji& Jiang, Xuheng& Zhang, Chao& Zhong, Jun& Fang, Xuanyu& Li, Huanhuan…[et al.]. 2020. Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation. Stem Cells International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1207759
Modern Language Association (MLA)
Zhang, Ji…[et al.]. Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation. Stem Cells International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1207759
American Medical Association (AMA)
Zhang, Ji& Jiang, Xuheng& Zhang, Chao& Zhong, Jun& Fang, Xuanyu& Li, Huanhuan…[et al.]. Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1207759
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207759