Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels
Joint Authors
Ahmad, Raja Elina
Pingguan-Murphy, Belinda
Nam, Hui Yin
Murali, Malliga Raman
Raghavendran, Hanumantha Rao Balaji
Kamarul, Tunku
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-18
Country of Publication
Egypt
No. of Pages
13
Abstract EN
It has been suggested that mechanical strain may elicit cell differentiation in adult somatic cells through activation of epithelial sodium channels (ENaC).
However, such phenomenon has not been previously demonstrated in mesenchymal stromal cells (MSCs).
The present study was thus conducted to investigate the role of ENaC in human bone marrow-derived MSCs (hMSCs) tenogenic differentiation during uniaxial tensile loading.
Passaged-2 hMSCs were seeded onto silicone chambers coated with collagen I and subjected to stretching at 1 Hz frequency and 8% strain for 6, 24, 48, and 72 hours.
Analyses at these time points included cell morphology and alignment observation, immunocytochemistry and immunofluorescence staining (collagen I, collagen III, fibronectin, and N-cadherin), and gene expression (ENaC subunits, and tenogenic markers).
Unstrained cells at similar time points served as the control group.
To demonstrate the involvement of ENaC in the differentiation process, an ENaC blocker (benzamil) was used and the results were compared to the noninhibited hMSCs.
ENaC subunits’ (α, β, γ, and δ) expression was observed in hMSCs, although only α subunit was significantly increased during stretching.
An increase in tenogenic genes’ (collagen1, collagen3, decorin, tenascin-c, scleraxis, and tenomodulin) and proteins’ (collagen I, collagen III, fibronectin, and N-cadherin) expression suggests that hMSCs underwent tenogenic differentiation when subjected to uniaxial loading.
Inhibition of ENaC function resulted in decreased expression of these markers, thereby suggesting that ENaC plays a vital role in tenogenic differentiation of hMSCs during mechanical loading.
American Psychological Association (APA)
Nam, Hui Yin& Murali, Malliga Raman& Ahmad, Raja Elina& Pingguan-Murphy, Belinda& Raghavendran, Hanumantha Rao Balaji& Kamarul, Tunku. 2020. Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels. Stem Cells International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1207788
Modern Language Association (MLA)
Nam, Hui Yin…[et al.]. Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels. Stem Cells International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1207788
American Medical Association (AMA)
Nam, Hui Yin& Murali, Malliga Raman& Ahmad, Raja Elina& Pingguan-Murphy, Belinda& Raghavendran, Hanumantha Rao Balaji& Kamarul, Tunku. Mechanical Strain-Mediated Tenogenic Differentiation of Mesenchymal Stromal Cells Is Regulated through Epithelial Sodium Channels. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1207788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207788