Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells
Joint Authors
Müller-Deubert, Sigrid
Ege, Carolin
Krug, Melanie
Meißner-Weigl, Jutta
Rudert, Maximilian
Bischof, Oliver
Jakob, Franz
Ebert, Regina
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-06
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Bone marrow-derived mesenchymal stromal cells (hMSCs) are capable of differentiating into the osteogenic lineage, and for osteogenic differentiation, mechanical loading is a relevant stimulus.
Mechanotransduction leads to the formation of second messengers such as cAMP, cGMP, or Ca2+ influx resulting in the activation of transcription factors mediating gene regulation.
The second messengers cAMP and cGMP are degraded by phosphodiesterase isoenzymes (PDE), but the role of these enzymes during osteogenic differentiation or mechanotransduction remains unclear.
Here, we focused on the isoenzyme phosphodiesterase 10A (PDE10A) and its role during osteogenic commitment and mechanotransduction.
We observed a time-dependent decrease of PDE10A expression in hMSC undergoing differentiation towards the osteogenic lineage.
PDE10A inhibition by papaverine diminished osteogenic differentiation.
While applying mechanical strain via cyclic stretching of hMSCs led to an upregulation of PDE10A gene expression, inhibition of PDE10A using the drug papaverine repressed expression of mechanoresponsive genes.
We conclude that PDE10A is a modulator of osteogenic differentiation as well as mechanotransduction in hMSCs.
Our data further suggests that the relative increase of cAMP, rather than the absolute cAMP level, is a key driver of the observed effects.
American Psychological Association (APA)
Müller-Deubert, Sigrid& Ege, Carolin& Krug, Melanie& Meißner-Weigl, Jutta& Rudert, Maximilian& Bischof, Oliver…[et al.]. 2020. Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1207869
Modern Language Association (MLA)
Müller-Deubert, Sigrid…[et al.]. Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1207869
American Medical Association (AMA)
Müller-Deubert, Sigrid& Ege, Carolin& Krug, Melanie& Meißner-Weigl, Jutta& Rudert, Maximilian& Bischof, Oliver…[et al.]. Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1207869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207869