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Spinal Reflex Recovery after Dorsal Rhizotomy and Repair with Platelet-Rich Plasma (PRP) Gel Combined with Bioengineered Human Embryonic Stem Cells (hESCs)
Joint Authors
Castro, Mateus Vidigal de
Silva, Moníze Valéria Ramos da
Chiarotto, Gabriela Bortolança
Santana, Maria Helena Andrade
Luzo, Ângela Cristina Malheiros
Kyrylenko, Sergiy
Oliveira, Alexandre Leite Rodrigues de
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-30
Country of Publication
Egypt
No. of Pages
16
Abstract EN
Dorsal root rhizotomy (DRZ) is currently considered an untreatable injury, resulting in the loss of sensitive function and usually leading to neuropathic pain.
In this context, we recently proposed a new surgical approach to treat DRZ that uses platelet-rich plasma (PRP) gel to restore the spinal reflex.
Success was correlated with the reentry of primary afferents into the spinal cord.
Here, aiming to enhance previous results, cell therapy with bioengineered human embryonic stem cells (hESCs) to overexpress fibroblast growth factor 2 (FGF2) was combined with PRP.
For these experiments, adult female rats were submitted to a unilateral rhizotomy of the lumbar spinal dorsal roots, which was followed by root repair with PRP gel with or without bioengineered hESCs.
One week after DRZ, the spinal cords were processed to evaluate changes in the glial response (GFAP and Iba-1) and excitatory synaptic circuits (VGLUT1) by immunofluorescence.
Eight weeks postsurgery, the lumbar intumescences were processed for analysis of the repaired microenvironment by transmission electron microscopy.
Spinal reflex recovery was evaluated by the electronic Von Frey method for eight weeks.
The transcript levels for human FGF2 were over 37-fold higher in the induced hESCs than in the noninduced and the wildtype counterparts.
Altogether, the results indicate that the combination of hESCs with PRP gel promoted substantial and prominent axonal regeneration processes after DRZ.
Thus, the repair of dorsal roots, if done appropriately, may be considered an approach to regain sensory-motor function after dorsal root axotomy.
American Psychological Association (APA)
Castro, Mateus Vidigal de& Silva, Moníze Valéria Ramos da& Chiarotto, Gabriela Bortolança& Santana, Maria Helena Andrade& Luzo, Ângela Cristina Malheiros& Kyrylenko, Sergiy…[et al.]. 2020. Spinal Reflex Recovery after Dorsal Rhizotomy and Repair with Platelet-Rich Plasma (PRP) Gel Combined with Bioengineered Human Embryonic Stem Cells (hESCs). Stem Cells International،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1207932
Modern Language Association (MLA)
Castro, Mateus Vidigal de…[et al.]. Spinal Reflex Recovery after Dorsal Rhizotomy and Repair with Platelet-Rich Plasma (PRP) Gel Combined with Bioengineered Human Embryonic Stem Cells (hESCs). Stem Cells International No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1207932
American Medical Association (AMA)
Castro, Mateus Vidigal de& Silva, Moníze Valéria Ramos da& Chiarotto, Gabriela Bortolança& Santana, Maria Helena Andrade& Luzo, Ângela Cristina Malheiros& Kyrylenko, Sergiy…[et al.]. Spinal Reflex Recovery after Dorsal Rhizotomy and Repair with Platelet-Rich Plasma (PRP) Gel Combined with Bioengineered Human Embryonic Stem Cells (hESCs). Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1207932
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207932