D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669bMAPK Pathway
Joint Authors
Jia, Lu
Xie, Chen
Hu, Lei
Liu, Yitong
Guo, Lijia
Fu, Jingfei
Jiang, Yiyang
Han, Nannan
Liu, Yi
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-10
Country of Publication
Egypt
No. of Pages
10
Abstract EN
The adipogenic differentiation of adipose tissue-derived stem cells (ADSCs) plays an important role in the process of obesity and host metabolism.
D-Mannose shows a potential regulating function for fat tissue expansion and glucose metabolism.
To explore the mechanisms through which D-mannose affects the adipogenic differentiation of adipose-derived stem cells in vitro, we cultured the ADSCs with adipogenic medium inducement containing D-mannose or glucose as the control.
The adipogenic differentiation specific markers Pparg and Fabp4 were determined by real-time PCR.
The Oil Red O staining was applied to measure the lipid accumulation.
To further explore the mechanisms, microarray analysis was performed to detect the differences between glucose-treated ADSCs (G-ADSCs) and D-mannose-treated ADSCs (M-ADSCs) in the gene expression level.
The microarray data were further analyzed by a Venn diagram and Gene Set Enrichment Analysis (GSEA).
MicroRNA inhibitor transfection was used to confirm the role of key microRNA.
Results.
D-Mannose intervention significantly inhibited the adipogenic differentiation of ADSCs, compared with the glucose intervention.
Microarray showed that D-mannose increased the expression of miR669b, which was an inhibitor of adipogenesis.
In addition, GSEA and western blot suggested that D-mannose suppressed the adipogenic differentiation via inhibiting the MAPK pathway and further inhibited the expression of proteins related to glucose metabolism and tumorigenesis.
Conclusion.
D-Mannose inhibits adipogenic differentiation of ADSCs via the miR669b/MAPK signaling pathway and may be further involved in the regulation of glucose metabolism and the inhibition of tumorigenesis.
American Psychological Association (APA)
Liu, Yitong& Guo, Lijia& Hu, Lei& Xie, Chen& Fu, Jingfei& Jiang, Yiyang…[et al.]. 2020. D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669bMAPK Pathway. Stem Cells International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1208017
Modern Language Association (MLA)
Liu, Yitong…[et al.]. D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669bMAPK Pathway. Stem Cells International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1208017
American Medical Association (AMA)
Liu, Yitong& Guo, Lijia& Hu, Lei& Xie, Chen& Fu, Jingfei& Jiang, Yiyang…[et al.]. D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669bMAPK Pathway. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1208017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208017