Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration
Joint Authors
Yu, Yan
Li, Na
Lu, Jiamin
Ge, Xingyun
Yu, Jinhua
Lei, Gang
Wang, Yanqiu
Li, Zehan
Manduca, Ana Gloria Cuba
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-19
Country of Publication
Egypt
No. of Pages
16
Abstract EN
Background.
Oral and maxillofacial bone loss is highly prevalent among populations, and nowadays, increased attention has been focused on dentin derivatives serving as desirable graft materials for bone regeneration.
In this study, dentin-derived inorganic mineral (DIM) was fabricated with a high-temperature calcination technique and the effects of DIM on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMMSCs) and the bone formation were elucidated.
Methods.
The effects of DIM on BMMSC proliferation and apoptosis capacity were evaluated by CCK-8, flow cytometry, and EdU assays.
Alkaline phosphatase (ALP) activity detection, ALP staining, alizarin red staining, and osteogenic marker expression analysis were performed to investigate the influence of DIM on the osteogenic differentiation of BMMSCs, as well as the relevant signal mechanisms.
The model of critical-sized defects in the calvarium of rats was constructed for exploring the in vivo efficiency of DIM on bone regeneration.
Results.
Cell viability assays indicated that DIM had no cytotoxicity.
BMMSCs cultured with DIM presented a higher level of osteogenic differentiation ability than those in the control group.
The activation in ERK and p38 signals was detected in DIM-treated BMMSCs, and both pathways and osteogenic process were suppressed while using ERK inhibitor U0126 and p38 inhibitor SB203580, respectively.
Furthermore, the animal experiments revealed that DIM could dramatically enhance new bone formation compared to the control group.
Conclusion.
DIM could promote BMMSC osteogenic differentiation via triggering the ERK and p38 MAPK signaling pathways and might be a novel predictable material for facilitating bone formation.
American Psychological Association (APA)
Lei, Gang& Wang, Yanqiu& Yu, Yan& Li, Zehan& Lu, Jiamin& Ge, Xingyun…[et al.]. 2020. Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration. Stem Cells International،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1208070
Modern Language Association (MLA)
Lei, Gang…[et al.]. Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration. Stem Cells International No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1208070
American Medical Association (AMA)
Lei, Gang& Wang, Yanqiu& Yu, Yan& Li, Zehan& Lu, Jiamin& Ge, Xingyun…[et al.]. Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1208070
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208070