Ascorbic Acid, Inflammatory Cytokines (IL-1βTNF-αIFN-γ), or Their Combination’s Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal StemProgenitor Cells
Joint Authors
Fawzy El-Sayed, Karim M.
Nguyen, Nhung
Dörfer, Christof E.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-17
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Objective.
Ascorbic acid (AA) and controlled inflammatory stimuli are postulated to possess the ability to independently exert positive effects on a variety of proliferative, pluripotency, and differentiation attributes of gingival mesenchymal stem/progenitor cells (G-MSCs).
The current study’s objective was to explore and compare for the first time the impact of the major inflammatory cytokines (IL-1β/TNF-α/IFN-γ), AA, or their combination on multipotency/pluripotency, proliferative, and differentiation characteristics of G-MSCs.
Design.
Human G-MSCs (n=5) were isolated and cultured in basic medium (control group), in basic medium with major inflammatory cytokines; 1 ng/ml IL-1β, 10 ng/ml TNF-α, and 100 ng/ml IFN-γ (inflammatory group), in basic medium with 250 μmol/l AA (AA group) and in inflammatory medium supplemented by AA (inflammatory/AA group).
All media were renewed three times per week.
In stimulated G-MSCs intracellular β-catenin at 1 hour, pluripotency gene expression at 1, 3, and 5 days, as well as colony-forming units (CFUs) ability and cellular proliferation over 14 days were examined.
Following a five-days stimulation in the designated groups, multilineage differentiation was assessed via qualitative and quantitative histochemistry as well as mRNA expression.
Results.
β-Catenin significantly decreased intracellularly in all experimental groups (p=0.002, Friedman).
AA group exhibited significantly higher cellular counts on days 3, 6, 7, and 13 (p<0.05) and the highest CFUs at 14 days [median-CFUs (Q25/Q75); 40 (15/50), p=0.043].
Significantly higher Nanog expression was noted in AA group [median gene-copies/PGK1 (Q25/Q75); 0.0006 (0.0002/0.0007), p<0.01, Wilcoxon-signed-rank].
Significant multilineage differentiation abilities, especially into osteogenic and chondrogenic directions, were further evident in the AA group.
Conclusions.
AA stimulation enhances G-MSCs’ stemness, proliferation, and differentiation properties, effects which are associated with a Wnt/β-catenin signaling pathway activation.
Apart from initially boosting cellular metabolism as well as Sox2 and Oct4A pluripotency marker expression, inflammation appeared to attenuate these AA-induced positive effects.
Current results reveal that for AA to exert its beneficial effects on G-MSCs’ cellular attributes, it requires to act in an inflammation-free microenvironment.
American Psychological Association (APA)
Fawzy El-Sayed, Karim M.& Nguyen, Nhung& Dörfer, Christof E.. 2020. Ascorbic Acid, Inflammatory Cytokines (IL-1βTNF-αIFN-γ), or Their Combination’s Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal StemProgenitor Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1208085
Modern Language Association (MLA)
Fawzy El-Sayed, Karim M.…[et al.]. Ascorbic Acid, Inflammatory Cytokines (IL-1βTNF-αIFN-γ), or Their Combination’s Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal StemProgenitor Cells. Stem Cells International No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1208085
American Medical Association (AMA)
Fawzy El-Sayed, Karim M.& Nguyen, Nhung& Dörfer, Christof E.. Ascorbic Acid, Inflammatory Cytokines (IL-1βTNF-αIFN-γ), or Their Combination’s Effect on Stemness, Proliferation, and Differentiation of Gingival Mesenchymal StemProgenitor Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1208085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208085