Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines
Joint Authors
Abdul Majid, A. M. S.
Yam, Mun Fei
Taleb Agha, Mohamad
Baharetha, Hussein M.
Al-Mansoub, Majed Ahmed
Tabana, Yasser M.
Kaz Abdul Aziz, Nur Hidayah
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-19
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In this study, the bioactivity-guided fractionation was conducted on the aerial parts of Arctium lappa L.
and then the extracts were tested in vitro on breast cancer (MCF-7), colorectal cancer (HCT-116), and normal cells (EA.hy926).
The n-hexane fraction (EHX) of the ethanolic extract showed strong activity against both MCF-7 and EA.hy926 cell lines (IC50 values: 14.08 ± 3.64 and 27.25 ± 3.45 μg/mL, respectively).
The proapoptotic activity of EHX was assessed using MCF-7.
Morphological alterations were visualized using Hoechst staining and a transmission electron microscope.
Cancer cell signal transduction pathways were investigated, and EHX significantly upregulated p53, TGF-β, and NF-κB.
Furthermore, EHX was found to disrupt the metastatic cascade of breast cancer cells by the inhibition of cell proliferation, migration, invasion, and colonization.
The antiangiogenic activity of EHX fraction showed potent inhibition of rat aorta microvessels with IC50 value: 4.34 ± 1.64 μg/mL.
This result was supported by the downregulation of VEGF-A expression up to 54%.
Over 20 compounds were identified in EHX using GC-MS, of which stigmasterol, ß-sitosterol, and 3-O-acetyllupeol are the major active compounds.
Phytochemical analysis of EHX showed higher phenolic and flavonoid contents with a substantial antioxidant activity.
In conclusion, this work demonstrated that A.
lappa has valuable anticancer activity and antiangiogenic properties that might be useful in breast cancer therapy.
American Psychological Association (APA)
Taleb Agha, Mohamad& Baharetha, Hussein M.& Al-Mansoub, Majed Ahmed& Tabana, Yasser M.& Kaz Abdul Aziz, Nur Hidayah& Yam, Mun Fei…[et al.]. 2020. Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines. Scientifica،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1208235
Modern Language Association (MLA)
Taleb Agha, Mohamad…[et al.]. Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines. Scientifica No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1208235
American Medical Association (AMA)
Taleb Agha, Mohamad& Baharetha, Hussein M.& Al-Mansoub, Majed Ahmed& Tabana, Yasser M.& Kaz Abdul Aziz, Nur Hidayah& Yam, Mun Fei…[et al.]. Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines. Scientifica. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1208235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208235