Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa
Joint Authors
Kim, Seung-Hun
Choi, Kwang-Hwan
Lee, Dong-Kyung
Lee, Mingyun
Hwang, Jae Yeon
Lee, Chang-Kyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-12
Country of Publication
Egypt
No. of Pages
11
Abstract EN
OCT4 plays pivotal roles in maintaining pluripotency during early mammalian embryonic development and in embryonic stem cells.
It is essential to establish a reporter system based on the OCT4 promoter region to study pluripotency.
However, there is still a lack of information about the porcine OCT4 upstream reporter system.
To improve our understanding of the porcine OCT4 regulatory region, we identified conserved regions in the porcine OCT4 promoter upstream region by sequence-based comparative analysis using various mammalian genome sequences.
The similarity of nucleotide sequences in the 5′ upstream region was low among mammalian species.
However, the OCT4 promoter and four regulatory regions, including distal and proximal enhancer elements, had high similarity.
Next, a functional analysis of the porcine OCT4 promoter region was conducted.
Luciferase reporter assay results indicated that the porcine OCT4 distal enhancer and proximal enhancer were highly activated in mouse embryonic stem cells and embryonic carcinoma cells, respectively.
A comparison analysis of naïve and primed state marker gene expression in a dual-reporter assay showed that the expression levels of naïve and primed markers differed in fluorescence signal between high-expressing cells and low-expressing cells.
Similar to OCT4 upstream-based reporter systems derived from other species, the porcine OCT4 upstream region-based reporter constructs showed exclusive expression patterns depending on the state of pluripotency.
This work provides basic information about the porcine OCT4 upstream region and various porcine OCT4 fluorescence reporter constructs, which can be applied to study species-specific pluripotency in early embryo development and the establishment of embryonic stem cells in pigs.
American Psychological Association (APA)
Kim, Seung-Hun& Choi, Kwang-Hwan& Lee, Dong-Kyung& Lee, Mingyun& Hwang, Jae Yeon& Lee, Chang-Kyu. 2019. Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa. Stem Cells International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1208347
Modern Language Association (MLA)
Kim, Seung-Hun…[et al.]. Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa. Stem Cells International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1208347
American Medical Association (AMA)
Kim, Seung-Hun& Choi, Kwang-Hwan& Lee, Dong-Kyung& Lee, Mingyun& Hwang, Jae Yeon& Lee, Chang-Kyu. Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1208347
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208347