Characteristics of Intestinal Microecology during Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury
Joint Authors
Cao, Hong-Cui
Ling, Zongxin
Dong, Xiaotian
Yu, Jiong
Liu, Jingqi
Pan, Qiaoling
Yang, Jinfeng
Feng, Xudong
Xu, Yanping
Li, Lanjuan
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-05
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Background.
The mechanisms of mesenchymal stem cell (MSC) transplantation to protect against acute liver injury have been well studied within the liver.
However, the associated changes in the intestinal microbiota during this process are poorly understood.
Methods.
In this study, compact bone-derived MSCs were injected into mice after carbon tetrachloride (CCl4) administration.
Potential curative effect of MSC was evaluated by survival rate and biochemical and pathological results.
Overall structural changes of microbial communities and alterations in the intestinal microbiota were assessed by sequenced 16S rRNA amplicon libraries from the contents of the cecum and colon.
Results.
MSCs significantly reduced the serum levels of aspartate transaminase and alanine transaminase and improved the histopathology and survival rate.
Lower expression and discontinuous staining of zonula occludens, as well as disrupted tight junctions, were observed in CCl4-treated mice at 48 h compared with MSC-transplanted mice.
Moreover, MSC transplantation to the liver leads to intestinal microbiota changes that were reflected in the decreased abundance of Bacteroidetes S24-7 and Bacteroidaceae and increased abundance of Firmicutes Clostridiales, Ruminococcaceae, and Lactobacillus at the initial time point compared with that in CCl4-treated mice.
In addition, phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt) based on the Greengenes database revealed functional biomarkers of MSC-transplanted mice involved in cell motility, signal transduction, membrane transport, transcription, and metabolism of lipids, cofactors, vitamins, terpenoids, and polyketides, as well as xenobiotics.
Conclusion.
The initial alterations in the Firmicutes/Bacteroidetes ratio, which resulted from MSC infusion to the liver, maintain intestinal mucosal biology and homeostasis that may be beneficial to liver repair.
American Psychological Association (APA)
Dong, Xiaotian& Feng, Xudong& Liu, Jingqi& Xu, Yanping& Pan, Qiaoling& Ling, Zongxin…[et al.]. 2019. Characteristics of Intestinal Microecology during Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury. Stem Cells International،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1208454
Modern Language Association (MLA)
Dong, Xiaotian…[et al.]. Characteristics of Intestinal Microecology during Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury. Stem Cells International No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1208454
American Medical Association (AMA)
Dong, Xiaotian& Feng, Xudong& Liu, Jingqi& Xu, Yanping& Pan, Qiaoling& Ling, Zongxin…[et al.]. Characteristics of Intestinal Microecology during Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1208454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208454