MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

Abstract EN

MicroRNAs (miRNAs) influence stem cell functions, including mobilization, proliferation, and differentiation.

miR-150 is abundantly expressed in monocytes.

Knockdown of miR-150 promotes bone marrow stem cell migration.

The role of miR-150 in adipose-derived stem cells (ADSCs) is unclear.

In this study, the effects of miR-150 on adipogenic differentiation and proliferation of ADSCs were investigated.

ADSCs were isolated from the inguinal adipose tissue of wild-type (WT) and miR-150 knockout (KO) mice and were induced for adipogenic differentiation.

The miR-150 level was detected by real-time PCR.

ADSCs were transfected by miR-150 or small-interfering RNA (siRNA) of Notch3.

MTT assay and colony formation assay were performed in miR-150 knockdown and control ADSCs.

Real-time PCR showed that miR-150 was expressed in ADSCs.

miR-150 knockdown significantly decreased the capacity of adipogenic differentiation of ADSCs, as compared with their counterparts from WT mice.

It is intriguing that the overexpression of miR-150 significantly increased C/EBPα and PPAR-γ expression and lipid formation in ADSCs with adipogenic induction.

Overexpression of miR-150 significantly decreased Notch3 expression in ADSCs compared with the control groups.

Furthermore, Notch3 inhibition promoted the adipogenic differentiation in ADSCs.

miR-150 also suppressed proliferation potential and the expression of Nanog in ADSCs.

In summary, this study demonstrates, for the first time, that miR-150 promotes adipogenic differentiation and inhibits proliferation of ADSCs.

miR-150 regulates adipogenic differentiation of ADSCs, likely mediated by the downregulation of Notch3.