Mesenchymal Stem Cells Attract Endothelial Progenitor Cells via a Positive Feedback Loop between CXCR2 and CXCR4
Joint Authors
Tan, Yingyun
Shu, Linjing
Xu, Peng
Bai, Shi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-05
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Mesenchymal stem cells (MSCs) can attract host endothelial progenitor cells (EPCs) to promote vascularization in tissue-engineered constructs (TECs).
Nevertheless, the underlying mechanism remains vague.
This study is aimed at investigating the roles of CXCR2 and CXCR4 in the EPC migration towards MSCs.
In vitro, Transwell assays were performed to evaluate the migration of EPCs towards MSCs.
Antagonists and shRNAs targeting CXCR2, CXCR4, and JAK/STAT3 were applied for the signaling blockade.
Western blot and RT-PCR were conducted to analyze the molecular events in EPCs.
In vivo, TECs were constructed and subcutaneously implanted into GFP+ transgenic mice.
Signaling inhibitors were injected in an orientated manner into TECs.
Recruitment of host CD34+ cells was evaluated by immunofluorescence.
Eventually, we demonstrated that CXCR2 and CXCR4 were both highly expressed in migrated EPCs and indispensable for MSC-induced EPC migration.
CXCR2 and CXCR4 strongly correlated with each other in the way that the expression of CXCR2 and CXCR2-mediated migration depends on the activity of CXCR4 and vice versa.
Further studies documented that both of CXCR2 and CXCR4 activated STAT3 signaling, which in turn regulated the expression of CXCR2 and CXCR4, as well as cell migration.
In summary, we firstly introduced a reciprocal crosstalk between CXCR2 and CXCR4 in the context of EPC migration.
This feedback loop plays critical roles in the migration of EPCs towards MSCs.
American Psychological Association (APA)
Tan, Yingyun& Shu, Linjing& Xu, Peng& Bai, Shi. 2019. Mesenchymal Stem Cells Attract Endothelial Progenitor Cells via a Positive Feedback Loop between CXCR2 and CXCR4. Stem Cells International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1208806
Modern Language Association (MLA)
Tan, Yingyun…[et al.]. Mesenchymal Stem Cells Attract Endothelial Progenitor Cells via a Positive Feedback Loop between CXCR2 and CXCR4. Stem Cells International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1208806
American Medical Association (AMA)
Tan, Yingyun& Shu, Linjing& Xu, Peng& Bai, Shi. Mesenchymal Stem Cells Attract Endothelial Progenitor Cells via a Positive Feedback Loop between CXCR2 and CXCR4. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1208806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208806