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Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells
Joint Authors
Hu, Tian
Xu, Yongde
Yao, Bin
Huang, Sha
Fu, Xiaobing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-04
Country of Publication
Egypt
No. of Pages
7
Abstract EN
Sweat glands developed from the embryonic epidermis.
To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed.
Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) using Transwell insert for evaluating the effects of soluble morphogens on sweat gland morphogenesis in vitro.
Using this coculture system, morphological alteration, histological features, and specific markers were observed.
Initial experiments revealed that ESCs cocultured with embryonic paw pad (EPP) tissue demonstrated glandular structure and cytokeratin 8 (K8) and cytokeratin 18 (K18) positive, while ESCs cocultured with embryonic dorsal skin demonstrated “sea snail” structure and K8, K18 negative.
Moreover, bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF) concentrations were detected in the medium of the EPP group.
BMP receptor inhibitor could effectively block the ESC differentiation to sweat glands, while EGF receptor blocker did not show the effect.
Our results showed clear benefits of this novel and convenient model in terms of in vitro-in vivo correlation.
It was an appropriate alternative for screening of potential bioactives regulating the sweat gland morphogenesis mechanism.
American Psychological Association (APA)
Hu, Tian& Xu, Yongde& Yao, Bin& Fu, Xiaobing& Huang, Sha. 2019. Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208824
Modern Language Association (MLA)
Hu, Tian…[et al.]. Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells. Stem Cells International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1208824
American Medical Association (AMA)
Hu, Tian& Xu, Yongde& Yao, Bin& Fu, Xiaobing& Huang, Sha. Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208824