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MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells
Joint Authors
Štefková, Kateřina
Kučera, Jan
Hanáčková, Markéta
Radaszkiewicz, Katarzyna Anna
Ambrůžová, Barbora
Pacherník, J.
Kubala, Lukas
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth factors important for haematopoiesis.
The role of p38alpha kinase was previously analysed in particular haematopoietic cells.
In this study and for the first time, the role of p38alpha kinase in haematopoiesis was studied using a model of continuous haematopoietic development in pluripotent embryonic stem cells in vitro.
The expression of transcripts associated with haematopoiesis and the potential for the formation of specific haematopoietic cell colonies were compared between wild-type and mutant p38alpha gene-depleted cells.
The absence of p38alpha kinase led to the inhibition of hemangioblast formation during the first step of haematopoiesis.
Later, during differentiation, due to the lack of p38alpha kinase, erythrocyte maturation was impaired.
Mutant p38α−/− cells also exhibited decreased potential with respect to the expansion of granulocyte colony-forming units.
This effect was reversed in the absence of erythropoietin as shown by colony-forming unit assay in media for colony-forming unit granulocytes/macrophages.
p38alpha kinase thus plays an important role in the differentiation of common myeloid precursor cells into granulocyte lineages.
American Psychological Association (APA)
Štefková, Kateřina& Hanáčková, Markéta& Kučera, Jan& Radaszkiewicz, Katarzyna Anna& Ambrůžová, Barbora& Kubala, Lukas…[et al.]. 2019. MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1208964
Modern Language Association (MLA)
Štefková, Kateřina…[et al.]. MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells. Stem Cells International No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1208964
American Medical Association (AMA)
Štefková, Kateřina& Hanáčková, Markéta& Kučera, Jan& Radaszkiewicz, Katarzyna Anna& Ambrůžová, Barbora& Kubala, Lukas…[et al.]. MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1208964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208964