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Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice
Joint Authors
Watanabe, Satoshi
Nakamura, Shingo
Sato, Masahiro
Saitoh, Issei
Inada, Emi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Isolation of hepatocytes and their culture in vitro represent important avenues to explore the function of such cells.
However, these studies are often difficult to perform because of the inability of hepatocytes to proliferate in vitro.
Immortalization of isolated hepatocytes is thus an important step toward continuous in vitro culture.
For cellular immortalization, integration of relevant genes into the host chromosomes is a prerequisite.
Transposons, which are mobile genetic elements, are known to facilitate integration of genes of interest (GOI) into chromosomes in vitro and in vivo.
Here, we proposed that a combination of transposon- and liver-directed introduction of nucleic acids may confer acquisition of unlimited cellular proliferative potential on hepatocytes, enabling the possible isolation of immortalized hepatocyte cell lines, which has often failed using more traditional immortalization methods.
American Psychological Association (APA)
Sato, Masahiro& Saitoh, Issei& Inada, Emi& Nakamura, Shingo& Watanabe, Satoshi. 2019. Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1208967
Modern Language Association (MLA)
Sato, Masahiro…[et al.]. Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1208967
American Medical Association (AMA)
Sato, Masahiro& Saitoh, Issei& Inada, Emi& Nakamura, Shingo& Watanabe, Satoshi. Potential for Isolation of Immortalized Hepatocyte Cell Lines by Liver-Directed In Vivo Gene Delivery of Transposons in Mice. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1208967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208967