Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage
Joint Authors
Raghavendran, H. Balaji
Malliga Raman, Murali
Vasudevaraj Naveen, Sangeetha
Elango, N.
Kamarul, Tunku
Baig, Salma
Azizan, Ainnul Hamidah Syahadah
Nam, Hui Yin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-26
Country of Publication
Egypt
No. of Pages
12
Abstract EN
We have determined the protective effects of Thymus serpyllum (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) in vitro.
Gas chromatography–mass spectroscopy confirmed the spectrum of active components in the extract.
Out of the three different extracts, the hexane extract showed significant free radical scavenging activity.
Treatment of MSCs with H2O2 (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200 μg/ml) suppressed H2O2-induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs.
H2O2-induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points (p<0.05).
H2O2 increased cell apoptosis.
In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably (p<0.05).
Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.
American Psychological Association (APA)
Baig, Salma& Azizan, Ainnul Hamidah Syahadah& Raghavendran, H. Balaji& Elango, N.& Vasudevaraj Naveen, Sangeetha& Malliga Raman, Murali…[et al.]. 2019. Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage. Stem Cells International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1208983
Modern Language Association (MLA)
Baig, Salma…[et al.]. Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage. Stem Cells International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1208983
American Medical Association (AMA)
Baig, Salma& Azizan, Ainnul Hamidah Syahadah& Raghavendran, H. Balaji& Elango, N.& Vasudevaraj Naveen, Sangeetha& Malliga Raman, Murali…[et al.]. Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1208983
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208983