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NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells
Joint Authors
Luedemann, Martin
Ziouti, Fani
Jundt, Franziska
Ebert, Regina
Rummler, Maximilian
Krug, Melanie
Müller-Deubert, Sigrid
Jakob, Franz
Willie, Bettina M.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-26
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Skeletal development and remodeling of adult bone are critically controlled by activated NOTCH signaling in genetically modified mice.
It is yet unclear whether NOTCH signaling is activated by mechanical strain sensed by bone cells.
We found that expression of specific NOTCH target genes is induced after in vivo tibial mechanical loading in wild-type mice.
We further applied mechanical strain through cyclic stretching in human bone marrow-derived mesenchymal stromal cells (BMSCs) in vitro by using a bioreactor system and detected upregulation of NOTCH target gene expression.
Inhibition of the NOTCH pathway in primary BMSCs as well as telomerase-immortalized human BMSCs (hMSC-TERT) through the gamma-secretase inhibitor GSI XII blocked mechanotransduction and modulated actin cytoskeleton organization.
Short-hairpin RNA gene silencing identified NOTCH2 as the key receptor mediating NOTCH effects on hMSC-TERT cells.
Our data indicate a functional link between NOTCH activation and mechanotransduction in human BMSCs.
We suggest that NOTCH signaling is an important contributor to molecular mechanisms that mediate the bone formation response to mechanical strain.
American Psychological Association (APA)
Ziouti, Fani& Ebert, Regina& Rummler, Maximilian& Krug, Melanie& Müller-Deubert, Sigrid& Luedemann, Martin…[et al.]. 2019. NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208997
Modern Language Association (MLA)
Ziouti, Fani…[et al.]. NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208997
American Medical Association (AMA)
Ziouti, Fani& Ebert, Regina& Rummler, Maximilian& Krug, Melanie& Müller-Deubert, Sigrid& Luedemann, Martin…[et al.]. NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208997